摘要
以中华猕猴桃伏牛95-2的组培苗叶片为外植体,研究了不同生长调节物质对不定芽的分化、增殖及生根的影响。结果表明:在MS+ZT1.0 mg/L+NAA0.3 mg/L培养基中,愈伤组织不定芽分化率最高,为87.5%;在MS+6-BA2.0 mg/L+NAA0.5 mg/L培养基中,不定芽增殖系数最高,为4.2;在1/2MS+NAA2.0 mg/L培养基中,不定芽生根率为100%。
Using the leaves of test-tube seedlings of Actinidia chinensis Funiu 95-2 as the explants,the effects of different growth regulating substances on the regeneration,proliferation and rooting of adventitious buds were researched.The results indicated that the highest callus inducement frequency（87.5%） was obtained on MS medium supplemented with 1.0 mg/L ZT and 0.3 mg/L NAA;the best medium for the proliferation of the adventitious buds was MS medium supplemented with 2.0 mg/L 6-BA plus 0.5 mg/L NAA,the proliferation coefficient was 4.2;the best medium for rooting of the adventitious buds was 1/ 2 MS medium with 2.0 mg/L NAA,the rooting rate was 100%.
出处
《江西农业学报》
CAS
2010年第4期50-52,共3页
Acta Agriculturae Jiangxi
关键词
中华猕猴桃
组织培养
植株再生
叶片
Actinidia chinensis
Tissue culture
Plant regeneration
Leaf
