摘要
采用硫酸铵二级盐析、330（OH）型树脂脱色、CM-52阳离子树脂离子交换层析三步法从发酵液中提取纳豆激酶。结果发现：（1）硫酸铵盐析采用20％～60％饱和度范围，纯化倍数为3．23，收率为72．36％；（2）330（0H）型树脂脱色，在脱色的同时可以除去杂蛋白，样品纯度达到电泳显示2条带：（3）CM．52阳离子交换树脂对纳豆激酶属于优吸型吸附，在pH6．0，0．01mol／L的PBS中交换效果最好，可以得到电泳纯的纳豆激酶，总收率为48．51％。
The extraction methods of nattokinase from fermentation fluid were studied with two degree saltout of （NH4）2SO4, decolor of resin 330 （OH） and positiveion resin CM-52 Ion exchange. As a result： （1） the saturation of （NH4）2SO4 saltingout was 20 %-60 %, purification multiple is 3.23, recovery was 72.36 %; （2） after resin 330 （OH） decolorization, the sample can achieve the purity of electrophoresis and demonstrate two belts; （3） positive ion resin CM-52 ion exchange adsorption was superior adsorption of nattokinase, the best exchange effect was with pH 6.0, 0.01 mol/L PBS, existing only one elution peak, it can dissolve the hitch and its purity of electrophoresis of nattokinase. The total recovery was 48.51%.
出处
《中国酿造》
CAS
北大核心
2010年第4期119-123,共5页
China Brewing
关键词
纳豆激酶
分离纯化
盐析
脱色
离子交换
nattokinase
separation and purification
salting-out
decoloratio
ion exchange
