摘要
目的采用2种不同方法建立实验性自身免疫性甲状腺炎的动物,比较两者的成功性、可靠性和可操作性。为研究自身免疫性甲状腺疾病的发病机制及其治疗提供有效的动物模型。方法2个实验组的大鼠分别使用单纯的碘剂喂养和碘剂联合抗原免疫,分别建立动物模型,分别进行甲状腺组织学病理改变的观察和血清中甲状腺自身抗体水平的测定。结果2个实验组与正常对照组相比,甲状腺组织中均可见炎症反应和炎症细胞浸润,血清中甲状腺自身抗体的水平有不同程度的升高;且碘剂联合抗原免疫组的甲状腺炎发病率、抗体水平均要高于单纯碘剂喂养组。结论采用碘剂联合抗原免疫所诱导的动物模型较好,与人类的桥本氏甲状腺炎具有相同的病理学基础与表现,能更好地应用于对自身免疫性甲状腺疾病的发病机制及其治疗的研究。
Objective To compare two different methods for establishing the model of experimental autoimmune thyroiditis （EAT）in rats and to provide an effective animal model for further investigating the pathogenesis and treatment of autoimmune thyroid diseases.Methods The experimental groups were respectively fed using iodine and iodine combined antigen immunization,then compare with the controls.The pathological of the thyroid and test the serum levels of thyroid autoantibodies were examined after experiment.Results The two experimental groups compared with the normal control group,inflammatory response and inflammatory cell infiltration was able to be seen in the thyroid tissue;the serum levels of thyroid autoantibodies with different degrees of increase.The different levels of thyroid autoantibodies also existed between the two experimental groups with statistical significance.Conclusion The experimental using iodine combined antigen-induced animal model with more effectiveness than other.It has the same pathological basis and performance with human Hashimoto＇s thyroiditis.It could be applied to the autoimmune thyroid disease pathogenesis and treatment research.
出处
《实用临床医学（江西）》
CAS
2010年第3期7-9,F0003,共4页
Practical Clinical Medicine
