摘要
目的观察硒干预后自身免疫性甲状腺炎(EAT)大鼠甲状腺超微结构改变,探讨硒对甲状腺自身免疫损伤反应的影响。方法建立自身免疫性甲状腺炎大鼠模型,对患病大鼠预防性和治疗性给予亚硒酸钠,观察其干预后甲状腺病理组织学变化和超微结构改变。结果实验结束时,硒预防EAT组和硒治疗EAT组TgAb、TmAb水平与EAT组相比明显下降(P<0.05),且光镜下炎性细胞浸润明显减少,滤泡破坏减轻。电镜下EAT组大鼠主要表现为部分滤泡上皮细胞断裂,内质网高度扩张水肿,线粒体明显减少,部分线粒体嵴消失。硒预防组和硒治疗组均可见滤泡形态较规则,内质网扩张程度减轻,线粒体增多且结构趋于正常。结论硒可预防和减轻自身免疫性甲状腺炎大鼠甲状腺的免疫性损伤。
Objective To observe the effects of selenium on the ultramicrostructure of thyroid of rats with autoimmune thyroiditis, and to study the influence of selenium on the autoimmune injury of thyroids. Methods The rat model of autoimmune thyoiditis was establish and treated with autoimmune thyroiditis with sodium selenite prophylacticly and remedially. The changes of pathohistology and ultramicrostructure of thyroid after intervention were observed. Results At the end of the experiment, the levels of TgAb and TmAb in selenium prophylaxis-EAT group and selenium curatio-EAT group were much lower than that of EAT group （ P 〈 0.05）. The infiltration of inflammnatory cell and the destroy of follicle were less serious in selenium group than those in EAT group. In the EAT group, the breakage of part of follicular epithelial ceils, the distension of endocytoplasmic reticulum, the decrease of chondriosome, and the disappearing of some chondriosome spine were observed. In selenium prophylaxis-EAT group and selenium curatio-EAT group, cryptae shape was much regular, the distension of endocytoplasmic reticulum was less serious, and chondriosome was much normal. Conclusion Selenium has prophylaxis and therapeutic effects on EAT rats.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2007年第5期528-530,534,共4页
Immunological Journal
关键词
硒
自身免疫性甲状腺炎
干预
超微结构
Selenium
Autoimmune thyroiditis
Intervention
Uhramicrostructure
