摘要
发展了一种基于双链荧光核酸适体（F-Aptamer）探针的简单快速检测蛋白质的分析方法.该双链荧光Aptamer探针由一条带荧光标记的Aptamer探针和带猝灭标记的互补DNA组成,当靶蛋白存在时,能形成比双链荧光Aptamer探针更稳定的F-Aptamer/蛋白质复合物,并发出荧光,从而实现对蛋白质的简便快速检测,检测线性范围为6~100 nmol/L,检出限为6 nmol/L.该方法设计简单,对核酸适体分子的大小和空间结构没有要求,可作为一种通用的基于F-Aptamer识别机理的蛋白质检测方法.
A simple method of protein detection was developed using a double-stranded fluorescent aptamer probe.This double-stranded aptamer probe consisted of an aptamer probe labeled with a fluorophore and a short complementary oligonucleotide labeled with a quencher.In the presence of protein,the double-stranded aptamer probe dissociated and F-aptamer/protein complex was formed,leading to fluorescence restoring.Then protein detection was carried out by monitoring fluorescence enhancement,with a liner range of 6—100 nmol/L and detection limit of 6 nmol/L.This design strategy is easy to generalize for any aptamer without prior knowledge of its secondary or tertiary structure,and would be used as a simple and general tool for protein detection.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2010年第2期274-278,共5页
Chemical Journal of Chinese Universities
基金
国家“九七三”计划项目(批准号:2002CB513110)
科技部国际合作重点项目(批准号:2003DF000039)
国家自然科学基金(批准号:90606003,20805012)
湖南省杰出青年科学基金(批准号:08JJ1002)资助
