摘要
目的鳗弧菌(Vibrio anguillarum)可引起鲑鱼、鳗鲡、鲈鱼和牙鲆等多种水产养殖动物的疾病,是水产养殖中的一种重要病原菌,对其进行快速检测是确保水产养殖安全和食品安全所必需的。方法本文利用鳗弧菌与其核酸适配体之间较强的亲和力,通过鳗弧菌夺取胶体金颗粒表面的核酸适配体,使胶体金溶液的吸光度发生变化,从而建立一种可定量检测鳗弧菌的方法。结果该方法对鳗弧菌的吸光度值显著高于对溶藻弧菌、铜绿假单胞菌、变形假单胞菌、嗜水气单胞菌和迟钝爱德华氏菌等非目标菌的吸光度值(P<0.01),并在1~105CFU/ml的检测范围内呈现较好的线性关系。用该方法对不同盐度和鱼体组织样品进行加标回收检测,结果显示回收率和相对标准偏差等指标都符合相应检测标准。结论该检测方法对鳗弧菌有较好的特异性,可用于水产品或食品中鳗弧菌的定量检测。
Objective Vibrio anguillarum is an important pathogen in aquaculture,which can infect salmon,eel,perch and flounder.Rapid detection is necessary to ensure aquaculture and food safety.Methods Based on the strong affinity between V.anguillarum and its aptamers,the bacterium can capture its aptamer from the surface of colloidal gold particles and change the absorbance of the colloidal gold solution.Thus,a colorimetric method for the quantitative detection of V.anguillarum was established.Results The optical density for V.anguillarum by the present method was much higher than those of non-target bacteria such as Vibrio alginolyticus,Pseudomonas aeruginosa,Pseudomonas plecoglossicida,Aeromonas hydrophila and Edwardsiella tarda(P<0.01).The detection method had good linear relationship in the range of 1-105 CFU/ml.The seawater samples with different salinities and fish body tissue samples were detected for the application,and the results showed that the recoveries and relative standard deviations of these samples were in line with the corresponding detection standards.Conclusion The present detection method has good specificity for V.anguillarum and can be used for the quantitative detection of the bacterium in aquatic products or food.
作者
黄将远
郑江
薄军
徐晓津
谭英
HUANG Jiang-Yuan;ZHENG Jiang;BO Jun;XU Xiao-Jin;TAN Ying(Fisheries College of Jimei University,Xiamen 361021,China;Engineering Research Center of the Modern Technology for Eel Industry,Ministry of Education,Xiamen 361021,China;Third Institute of Oceanology,Ministry of Natural Resources,Xiamen 361005,China)
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2023年第9期2195-2204,共10页
Progress In Biochemistry and Biophysics
基金
福建省自然科学基金(2021J01823)
鳗鲡现代产业技术教育部工程研究中心开放基金(RE202205,RE202308)资助项目。
关键词
鳗弧菌
核酸适配体
亲和特异性
吸光度
Vibrio anguillarum
aptamer
affinity specificity
absorbance
