摘要
目的:对2008年崇明地区发生的霍乱疫情中分离的和平时监测中分离的霍乱菌株进行遗传相关性分析,追溯传染源,为霍乱疫情的预测和制定防治措施提供依据。方法:PCR检测霍乱弧菌毒力基因;脉冲场凝胶电泳(PFEG)对实验菌株进行分子分型,并分析结果。结果:所试8株菌株均为毒力阳性,均为产毒株。8株菌的PFGE为5个型。结论:8株O139群霍乱弧菌均为产毒株,具有致病性。崇明地区首次在被污染的苍蝇中分离出霍乱弧菌,经流调排除其为传染源,但要加强该环节的监测力度。2008年度崇明发生的4起霍乱疫情从分子生物学角度分析为4起独立散发的疫情,无关联性。
Objective：To study the genetic correlation of cholera strains isolated form cholera epidemic and environment by routine surveillance in 2008 in Chongming,to trace source of infection,provide a basis for predicting infectiongs and making cholera control measures.Methods：Cholera toxin gene waa detected by PCR,and the molecular types of strains were classified by Pulsed-Field Gel Electrophoresis（PFGE）.Results：CT gene was identified in all 8 strains of O139 vibrio cholera and 8 strains were clustered into 5 PFGE patterns.Conclusion：The 8 strains all were toxigenic and pathogenic.It′s the first time isolated Vibrio cholerae in flies in Chongming.We should strengthen surveillance for flies.Chongming annual occurrence of 08 cases of cholera epidemic of 4 from the perspective of molecular biology analysis of 4 independent distributed epidemic,no relevance.
出处
《中国卫生检验杂志》
CAS
2009年第12期2849-2850,共2页
Chinese Journal of Health Laboratory Technology
