摘要
[目的]研究不同碳源对虎眼万年青离体生长的影响。[方法]以虎眼万年青子鳞茎为外植体,以MS培养基为基本培养基,附加6-BA1.0mg/L、NAA0.2mg/L,在培养基中分别加入不同浓度的蔗糖(15、30、45g/L)、麦芽糖(15、30、45g/L)和葡萄糖(15、30、45g/L)。[结果]蔗糖为虎眼万年青离体生长的最佳碳源。当蔗糖浓度为30g/L时,虎眼万年青离体生长的繁殖系数最高,长势最好。[结论]为从根本上解决虎眼万年青生产过程中优化品种、周年供应、控制成本等问题提供理论依据。
[ Objective] The research aimed to study the effects of different carbon sources on in vitro growth of Ornithogalum dubium. [ Method ] Sub-bulb of Ornithogalum dubium was chose as explants and cultured on MS medium added 6-BA 1.0 nag/L, NAA 0.2 mg/L. Sucrose （ 15, 30, 45 g/L）, malt sugar （15, 30, 45 g/L） and glucose （15, 30, 45 g/L） were added in the MS medium to study the growth of Ornithogalum dubium. [ Result ~ The results showed that sucrose was the best carbon source of in vitro growth of Ornithogalum dubium. When the sucrose concentration was 30 g/L, the reproduction coefficient of Ornithogalum dubium＇s in vitro growth was the highest, the growing trend was the best. [ Conclusion] The study can provide theoretic basis for fundamentally settling the problems of optimizing variety, annual supply and control cost and so on of Ornithoealum dubium.
出处
《安徽农业科学》
CAS
北大核心
2009年第34期16767-16768,共2页
Journal of Anhui Agricultural Sciences
基金
吉林省教育厅项目(2007426)
关键词
虎眼万年青
碳源
离体生长
Ornithogalum dubium
Carbon source
in vitro growth
