摘要
考察液体发酵培养基中不同碳源、碳源用量以及培养基pH值对银耳菌丝体富集酸性多糖的影响。结果表明:1%的蔗糖、pH值为7.0的培养基有利于银耳菌丝体酸性多糖的富集。并对所得银耳多糖进行DEAE-sepherose fast flow柱层析,发现菌丝体与子实体相比,多糖组成发生显著改变。
This paper mainly deals with effects of different carbon sources, carbon source amount and medium pH on accumulation of acidic polysaccharides in Tremellafuciformis mycelia during submerged fermentation. Along with this, the fractionation of the polysaccharides by DEAE Sepharose Fast Flow chromatography is also discussed in this paper. Results showed that the fermentation medium containing 1% sucrose at pH 7.0 was conducive to the enrichment of acidic polysaccharides. The distribution of polysaccharide in mycelia and fruit body of Tremellafuciformis revealed a significant change.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第21期175-177,共3页
Food Science
基金
四川省中医药管理局科技专项资助项目(2008-01)
四川省教育厅科研项目(08ZA173)
关键词
银耳
菌丝体
酸性多糖
液体深层发酵
Tremella fuciformis
mycelium
acidic polysaccharide
submerged fermentation
