摘要
采用酚仿法、CTAB法、蛋白酶K法、氯化苄法和盐析法五种方法分别提取银耳芽孢基因组DNA,用吸光度估计DNA的纯度和得率、RAPD(随机扩增多态DNA)技术和限制性内切酶酶切反应评价DNA的质量。结果表明:五种方法所提的DNA纯度及产率有差别,氯化苄法和蛋白酶K法的产率要高于另外三种方法,但CTAB法所提DNA纯度最高。综合而言,CTAB法是五种方法中最为适合银耳芽孢基因组DNA提取的方法。
DNA was extracted from spores of Tremellafuciformis by phenol-chloroform method, CTAB （cetyltrimethyl ammonium bromide） method, proteinase K method, benzyl chloride method and salting out method respectively. The purity and yield of the DNA were determined by A260/A280 respectively. The quality of the DNA were evaluated with RAPD （random amplified polymerphic DNA technology） and restrict endonuclease digestion. Results showed that there are some differences in purity and yield among those 5 methods. Both benzyl chloride method and proteinase K method have better yield, but the purity of the DNA by CTAB method is better than that of others. In a word, the CTAB method is most suitable for extracting genomic DNA from spores of Tremellafuciformis.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第6期248-251,共4页
Food Science
基金
中国科学院微生物研究所资源中心资助项目
关键词
银耳
芽孢
DNA提取
RAPD
限制性内切酶酶切
Tremella fuciformis
spores
DNA extraction
RAPD
restriction endonuclease digestion
