摘要
目的探讨乳腺癌组织中WT1基因表达水平及其临床意义。方法采用实时定量RT—PCR方法，检测110例乳腺肿瘤及其邻近正常乳腺组织中的WT1基因及内参照GAPDH的表达水平，以WT1N=（WT1拷贝数／GAPDH拷贝数）×10^4计算WT1表达水平，并计算同一患者肿瘤组织与正常组织中WT1。比值，定义为T／NWT1比值，分析WT1基因表达（T／NWT1）水平与临床病理参数的关系。结果102例乳腺癌组织中WT1N明显高于其周围邻近正常乳腺组织中的表达（P〈0．01），而8例乳腺良性肿瘤组织与其相应的正常乳腺组织中WT1N表达水平差异无统计学意义（P〉0．05）。乳腺癌组织中WT1N及其T／NWT1比值明显高于乳腺良性肿瘤组织（P〈0．01），且T／NWT1比值与有无淋巴结转移、乳腺癌病理类型、雌激素受体（ER）状态及孕激素受体（PR）状态无相关性。WT1基因表达水平与IL-8基因表达水平具有很好的相关性（r=0．723，P〈0．01）。结论乳腺癌组织高表达WT1，乳腺癌组织中WT1表达水平可作为评价疾病进展及判断预后的指标。
Objective To investigate the clinical significance of Wilm tumor gene （WT1） expression in breast cancer. Methods Real-time quantitative reverse transcriptase polymerase chain reaction （RQ-RT- PCR） method was established for detecting WT1 and GAPDH mRNA expression levels in 110 cases of various breast tumor and the corresponding adjacent normal breast tissue. Normalized WT1 expression level （WT1N） was determined as a ratio between WT1 and GAPDH for each case. The tumor tissue WT1N over the normal tissue WT1N of the same case was calculated as T/Nwn ratio, and T/N^I value was analyzed with the clinical- pathological parameters. Results The WT1N expression levels of the 102 breast cancer tissues were significantly higher than those of the adjacent normal breast tissues, with the median WT1N of 2.38 （ranged from 0.12 to 112.3） and 0.81 （ranged from 0.03 to 11.65） for each （P 〈0.01）, but there were no statistical differences between the WT1N of 8 benign breast tumors and the nearing normal tissues, with the median WT1N of 0.46 （ranged from 0.16 to 5.04） and 0.53 （ranged from 0.14 to 4.94） for each. Furthermore the WT1N as well as the T/NWT1 ratio of the malignant breast cancer tissues were significantly higher than those of the benign tumor tissues, with the median T/NWT1 value of 2.54 （ranged from 0.28 to 172.88） and 1.17 （ranged from 0.09 to 2.63） for each. Non-parameter correlation analysis showed that the T/NWT1 in breast cancers were of no relevance to lymph node metastasis, clinical-pathological types, estrogen receptor and progestone receptor status, but positively correlated with the expression level of IL-8 gene which calculated with T/N IL-8 （r =0.723,P 〈0.01）. Conclusion The WT1 gene is highly expressed in breast cancer which suggests that WT1 level assessed by RQ-RT-PCR could be a novel marker of disease progression and poor prognosis.
出处
《肿瘤研究与临床》
CAS
2009年第11期731-733,736,共4页
Cancer Research and Clinic
