摘要
目的:探讨慢性间歇低氧对幼鼠海马神经细胞凋亡及caspase-12表达的影响。方法:取体重为80～100g健康雄性SPF级SD幼鼠(3～4周龄)40只,随机分为间歇低氧2周(2IH)组、4周(4IH)组,空气对照2周(2C)组、4周(4C)组,每组10只。以间歇低氧舱建立慢性间歇低氧幼鼠模型。采用TUNEL法检测海马神经细胞凋亡,RT-PCR检测海马区caspase-12 mRNA表达,免疫组织化学染色检测海马区caspase-12蛋白的表达。结果:间歇低氧组凋亡指数(AI)、caspase-12 mRNA及其蛋白表达均高于对照组(均P<0.01);4IH的AI,caspase-12 mRNA及其蛋白表达均高于2IH(均P<0.01)。结论:慢性间歇低氧幼鼠海马神经细胞caspase-12 mRNA及其蛋白表达随间歇低氧时间延长而增加,内质网应激参与慢性间歇低氧幼鼠海马神经细胞凋亡过程。
Objective： To explore the effect of chronic intermittent hypoxia on apoptosis of hippocampal cells and the expression of caspase-12 in immature rat’s. Methods： Forty male Sprague- Dawley rats （3～4 week,80～100 g） were randomly divided into four groups： 2-week intermittent hypoxia group （2IH）,4-week intermittent hypoxia group （4IH）,2-week control group with room air（2C） and 4-week control group with room air （4C）, and ten rats in each group. Intermittent hypoxia model was induced by an intermittent hypoxia cabin,the in situ cell apoptosis in the hippocampus was detected with TUNEL staining. The expressions of caspase-12mRNA in the hippocampus were examined with RT-PCR. Immunohistochemical SP method was used to examine the expression of caspase-12 in the hippocampus. Results： Both the apoptosis index（AI）and the expressions of caspase-12 in hippocampus of intermittent hypoxia group were significantly higher than those in control group （P 〈0.01）. Increasing the duration of hypoxic period. , 4IH group had significantly higher levels of AI and the expression of caspase-12 than that of 2IH group. Conclusion： The expression of caspase-12 increases significantly and it shows that endoplasmic reticulum stress plays an important role in the course of apoptosis of immature rat’s hippocampal cells induced by chronic intermittent hypoxia.
出处
《温州医学院学报》
CAS
2009年第5期422-425,共4页
Journal of Wenzhou Medical College
基金
浙江省医药卫生科学研究基金资助项目(2006B104)
浙江省科技厅新苗人才计划资助项目(2007G60G2090029)
温州市科技局科研基金资助项目(Y2006A007)
