摘要
目的观察葡萄籽来源的原花青素(grape seed proanthocyanidin extract,GSPE)对人卵巢癌耐药细胞COC1/DDP在体外的增殖抑制作用,并初步探讨其作用机制。方法以MTT法检测不同浓度的GSPE(0、20、40、80、160、320μg/ml)不同时间(12、24、36、48、72h)对COC1/DDP的增殖抑制作用,HE染色观察细胞形态变化,流式细胞仪和TUNEL检测细胞周期和凋亡,Westernblot检测细胞内Caspase-3蛋白的表达。结果GSPE对COC1/DDP具有明显的增殖抑制作用,且呈剂量-时间依赖性,不同浓度GSPE能引起细胞形态明显的凋亡改变。流式细胞术和TUNEL表明GSPE可以诱导细胞凋亡,且随着药物浓度增加,凋亡率升高。Westernblot检测显示随着药物浓度增加,Caspase-3蛋白表达增加。结论原花青素可以在体外抑制人卵巢癌耐药细胞COC1/DDP的增殖并诱导其凋亡,其机制可能与上调Caspase-3蛋白表达有关。
Objective To investigate the effects of grape seed proanthocyanidin extract （ GSPE） on the proliferation and apoptosis of cisplatin-resistant human ovarian cancer cell line COC1 /DDP and explore the implying molecular mechanism. Methods COC1 /DDP cells were cultured in the presence of GSPE （ at 0, 20,40,80,160 or 320 μg/ml） for 1,24,36,48 or 72 h of culture. The morphological changes of COC1 / DDP were observed with HE staining,and the proliferation was measured by MTT assay and cell apoptosis by flow cytometry and TUNEL. Western blot analysis were performed to detect the protein expression of Caspase-3. Results GSPE inhibited the growth of COC1 /DDP cells in a concentration-and time-dependent manner. Flow cytometry analysis and TUNEL showed that GSPE induced apoptosis of COC1 /DDP cells and the percentage of apoptotic cells was increased with the concentration of GSPE administered and also the elongation of treatment time. The expression of caspase-3 was upregulated in the course of COC1 /DDP cell apoptosis when treated with GSPE. Conclusion GSPE inhibits the proliferation and induces cell apoptosis in the human ovarian cancer cell lines COC1 /DDP ,probably through upregulating caspase-3 expression.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2009年第21期2124-2127,共4页
Journal of Third Military Medical University
基金
重庆医科大学校级科研课题(XBYB2008035)~~
