摘要
目的:观察双氢青蒿素对SKOV3/CDDP细胞的体外作用,分析双氢青蒿素抗肿瘤和逆转耐药的效果。方法:采用MTT法观察顺铂、双氢青蒿素以及顺铂联合双氢青蒿素对SKOV3及SKOV3/CDDP细胞的体外影响。结果:①顺铂对SKOV3/CDDP细胞的IC50为34.12mg/L,是对SKOV3细胞的(IC50为7.52mg/L)4.54倍。②不同浓度的双氢青蒿素对两种细胞的抑制作用随浓度的增加而增强,呈明显的剂量依赖性,组间比较差异有统计学意义(P<0.05)。双氢青蒿素对SKOV3细胞的IC50为27.45μmol/L,对SKOV3/CDDP细胞的IC50为29.22μmol/L。③不同浓度的双氢青蒿素联合顺铂作用于SKOV3细胞,顺铂IC50由单用时的7.52mg/L,下降到联用时的5.30mg/L和2.16mg/L。对SKOV3/CDDP细胞,顺铂IC50由单用时的34.12mg/L,下降为到联用时的15.25mg/L和4.83mg/L。结论:双氢青蒿素对人卵巢癌顺铂耐药细胞SKOV3/CD-DP具有体外抑制作用,对顺铂具有化疗增敏的作用,并能逆转SKOV3/CDDP对顺铂的耐药。
Objective：The effect of dihydroartemisinin and cisplatin on human ovarian cancer cells SKOV3 and SKOV3/CDDP in vitro was observed inhibition cancer cell and reversion chemoresistance of dihydroartemisinin was evaluated.Methods：The effects of single cisplatin,single dihydroartemisinin and two combined drugs on ovarian cancer cell lines SKOV3,SKOV3/CDDP in vitro was measured by MTT assay.Results：① IC50 of cisplatin on SKOV3/CDDP was 34.12 mg/L which was 4.54 times of IC50 on SKOV3（7.52 mg/L）.② The inhibition function was augmented by increased concentration of Dihydroartemisinin which was dose dependence,the differences were significant among different concentration groups（P〈0.05）.IC50 of dihydroartemisinin on SKOV3 and SKOV3/CDDP was 27.45 μmol/L and 29.22 μmol/L.③ IC50 of cisplatin when combined with different doses of dihydroartemisinin on SKOV3 was dropped from original 7.52 mg/L to 5.30 mg/L and 2.16 mg/L.IC50 of cisplatin in combined mannerson SKOV3/CDDP was dropped from original 34.12 mg/L to 15.25 mg/L and 4.83 mg/L.Conclusions：Dihydroartemisinin can inhibit the proliferation of SKOV3/CDDP in vitro,augment the chemotherapy function of cisplatin,and reverse cisplatin resistance.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2010年第5期348-351,共4页
Journal of Practical Obstetrics and Gynecology
关键词
双氢青蒿素
卵巢癌
体外抑制
Dihydroartemisinin
Ovarian cancer
Inhibition in vitro
