摘要
以乙醇作溶剂,水浴加热回流提取雪莲果中的绿原酸,于紫外可见分光光度计上进行扫描。对比绿原酸标准品和雪莲果提取液的紫外扫描图谱,发现在330nm处都有一个最大吸收峰。在单因素试验的基础上确定以乙醇浓度、提取温度、提取时间、物料比作为试验因子进行L(934)正交试验。绿原酸标准品标准工作曲线的线性方程:A=-0.12575+59.18527C,相关系数r=0.9997。通过正交试验,确定雪莲果中绿原酸的最佳提取条件为:乙醇质量分数为40%,物料比为1∶18(g/mL),于沸水浴中回流提取时间为2.5h。在最佳工艺条件下测得雪莲果中绿原酸含量为0.052%。
To make ethanol solvent,the experiments were conducted with extraction chlorogenic acid from smallanthus sonchifolius by water bath heating and scaned in the UV-Vis spectrophotometer. Chlorogenic acid and the Standard of UV scanning maps were found that there was a maximum absorption peak in the 330 nm. With the single-factor experiment, using L9 （34） o rthogonal design, the influences of ethanol concentration, temperature, the time, the ratio of material to liquid were studied. Chlorogenic acid standard curve of standard linear equation： A = -0.125 75 ＋59.185 27 C, the correlation coefficient r = 0.999 7. The test result showed that, ethanol concent 40 %, boiling water bath, the ratio of material to liquid 1：18 （g/mL） extracts 2.5 h, chlorogenic acid extraction efficiency is the highest.Under this condition, chlorogenic acid rate is 0.052 %.
出处
《食品研究与开发》
CAS
北大核心
2009年第10期89-92,共4页
Food Research and Development
基金
红河学院科研项目(XJIY0706)
关键词
雪莲果
绿原酸
提取工艺
smallanthus sonchifolius
chlorogenic acid
extraction technology
