摘要
用FastPCR软件在大豆疫霉全基因组中搜索到1 234个含2～4个重复基元精确SSRs。选择260个SSRs设计引物,经对大豆疫霉5个分离物的基因组DNA检测,有212对(81.5%)有效扩增出SSR特征条带,112对(52.8%)扩增多态性。用18对多态性SSR引物分析了来自美国、中国黑龙江省和福建省大豆疫霉分离物的遗传多样性,在73个分离物中共扩增出112个等位变异,变异范围为4～9,平均为6.22个,表明选择的引物对具有高的多态性。在3个大豆疫霉群体中,黑龙江省和福建省分离物的遗传距离最近,美国和福建省分离物的遗传距离最远。UPGMA聚类将73个分离物划分为6组,其中8个美国分离物(72.73%)和53个中国分离物(85.48%)被聚类在一起,表明大豆疫霉中国分离物与美国分离物可能具有共同的祖先,中国分离物可能为外来种。
Using FastPCR software, 1 234 perfect simple sequence repeats （SSRs） with 2 to 4 bp repeat motif were identified in complete genomic sequences of Phytophthora sojae. 260 SSRs were selected for primer design, and the validity of resulting primer pairs was detected by amplifying 5 isolates of P. sojae. There were 212 （81.5%） primer pairs amplified characteristic SSR bands and 112 primer pairs （52.8%） amplified poly- morphic bands among 5 different P. sojae isolates. 18 primer pairs were selected to analyze the genetic diver- sity of 73 P. sojae isolates came from USA, Heilongjiang Province and Fujian Province of China. On the 18 SSR loci, a total of 112 alleles were detected in 73 isolates. At each locus,4 to 9 alleles were detected with an average of 6.22. The results showed that the selected primer pairs were high polymorphic. There was the closest genetic distance between the P. sojae populations from Heilongjiang and Fujian, while the populations from USA and Fujian had the farthest genetic distance. 73 P. sojae isolates could be divided into 6 groups by UPGMA clustering, 8 USA isolates （72.73 % ） were clustered together with 53 Chinese isolates （85.48 % ）, implicated that Chinese P. sojae isolates and some USA isolates should have several common ancestors and Chinese isolates could be exotic species.
出处
《植物病理学报》
CAS
CSCD
北大核心
2009年第4期337-346,共10页
Acta Phytopathologica Sinica
基金
“十一五”国家科技支撑计划(2006BAD08A08,2006BAD08A15)
国家重点基础研究发展计划“973”(2002CB111406)
公益性行业(农业)科研专项(3-20)
