摘要
采用Zeta电位和粒度分析仪测量了溶菌酶的水力学直径,研究了pH值、离子强度和脲浓度对其大小的影响。随着pH值增加,溶菌酶的水力学直径呈"W"形分布;在极端的碱性条件下,溶菌酶水力学直径随离子强度的增加而显著变大;中性pH值时,水力学直径随离子强度的增加变化不大。脲对溶菌酶具有双重作用,随着溶液中脲浓度的增加,溶菌酶的水力学直径先减小后增大。结果表明,动态光散射技术可以很好地应用于研究蛋白质分子的均一性和稳定性,同时也可以通过水力学直径来表征pH值、离子强度和脲对溶菌酶的影响。
The hydrodynamic diameter （Dh） of lysozyme was measured by Zetasizer Nano instrument. The influences of pH value, ionic strength and urea concentration on Dh of lysozyme were investigated. With pH value increasing, the Dh of lysozyme showed its special form of “W”. In the extreme alkaline solution, the Dh of lysozyme rose obviously with ionic strength. Ionic strength did not affect the Dh of lysozyme at neutral condition, under which its structure was steady and tight. The effect of urea on the Dh of lysozyme was complex relatively. Duality of urea played an important role in the denaturation of lysozyme. The Dh of lysozyme decreased first and then increased gradually with urea concentration. The results indicated that dynamic light scattering （DLS） was a convenient way to measure homogeneity and stability of the protein molecule, meanwhile Dh could be used for characterizing the influences of pH value, ionic strength and urea.
出处
《化学与生物工程》
CAS
2009年第9期85-87,共3页
Chemistry & Bioengineering
基金
国家自然科学基金资助项目(20672086)
西北工业大学科技创新基金资助项目(2008KJ02038)
关键词
溶菌酶
动态光散射技术
水力学直径
离子强度
脲
lysozyme
dynamic light scattering
hydrodynamic diameter
ionic strength
urea
