摘要
目的:建立测定益智挥发油中p-聚伞花烃含量的方法。方法:采用气相色谱法,HP-5弹性石英毛细管色谱柱(30m×0.32mm×0.25μm),载气为N2,流速1mL·min-1,进样口温度200℃,检测器温度280℃,程序升温,外标法计算含量。结果:p-聚伞花烃在0.43～4.30mg·mL-1范围内线性关系良好(r=0.9999);平均加样回收率为97.1%,RSD(n=6)为0.6%。结论:本方法简单、灵敏,重复性好,可用于益智挥发油中p-聚伞花烃的含量测定。
Objective： To establish a method for the determination of the content of p-cymene in volatile oil from Fruits of Alpinia oxyphylla. Methods： The Gas chromatographic analysis was performed using HP-5 capillary column（30m×0.32mm×0.25μm）; highly pure N2 was adopted as carrier gas at the flow rate of 1mL？min-1; the inlet port and the FID detector temperature was 200℃ and 280℃ respectively; the column temperature was 60-160℃ and rose by program. The content was calculated with external standard. Results： p-cymene has good liner relationship in the concentration range of 0.43-4.30 mg？mL-1（r=0.9999）; the average recovery was 97.1%, RSD（n=6） was 0.6%. Conclusion： The method is simple, accurate and reproducible. It can be applied for the determination of the content of p-cymene in volatile oil from Fruits of Alpinia oxyphylla Miq..
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第8期1333-1335,共3页
Chinese Journal of Pharmaceutical Analysis
基金
海南省自然科学基金(30629)
