摘要
目的:建立六味安消片中大黄素和大黄酚的含量测定方法。方法:用 HPLC 方法对制剂中大黄中所含的大黄素和大黄酚进行含量测定研究。采用 Inertsil ODS-3(5μm,250mm×4.6mm)柱,以甲醇-0.1%磷酸水溶液(85:15)为流动相,检测波长为254nm。结果:大黄素在0.0144～0.072μg范围内呈线性关系(r=0.9997,n=5),平均回收率为97.24%;大黄酚在0.035～0.175μg范围内呈线性关系(r=0.9999,n=5),平均回收率为99.35%。结论:本方法操作简便、可靠,重现性好,专属性强,可作为六味安消片的内在质量控制方法。
Objective ：To establish a HPLC method for determination of emodin and chrysophanol in Liuweianxiao tablets. Method：The chromatographic column was Inertsil ODS -3 （5 μm, 250 mm × 4. 6 mm）, UV detection wavelength was 254 nm, a mixture of 0. 1% phosphate - water and methanol was used as mobile phase. Results： Emodin at the quality of 0. 0144 -0. 072 μg showed good linearity, the average recovery was 97. 24% ; chrysophanol at the quality of 0. 035 -0. 175μg showed good linearity,the average recovery was 99.35%. Conclusion：This methods is fast, reliable and accurate to examine and may be as one of index used to judge the quality of Liuweianxiao tablets.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第7期1096-1099,共4页
Chinese Journal of Pharmaceutical Analysis
