摘要
目的了解河北省石家庄市不同感染人群人类免疫缺陷病毒I型(HIV-1)亚型及分布特点。方法从石家庄市报告的148例HIV-1抗体阳性者抗凝全血中提取前病毒DNA,用套式聚合酶链反应(nested-PCR)扩增病毒膜蛋白(ENV)基因的C2-V3区并进行序列测定,与国际标准参考序列比较确定亚型。结果148例样品分属8个亚型,45例为欧美B,37例为CRF07-BC,35例为泰国B(B'),25例为CRF01-AE,C和CRF02-AG各2例,CRF03-AB和CRF11-CPX各1例。结论既往供受血人员和注射吸毒人群感染的HIV-1亚型相对单一,性传播亚型分布较广。
Objective To investigate the subtypes and distribution characteristics of HIV-1 among different populations infected. Methods HIV-1 pro-viral DNA was extracted from the whole blood of 148 HIV-1 infected individuals. The HIV-1 env C2-V3 gene region was amplified by nested-PCR and was sequenced. The sequence was compared with HIV-1 subtype reference sequence. Results There were 8 kinds of HIV- 1 subtypes in 148 samples. In the subjects,45 cases were B subtype;37 were CRF07-BC;35 were B＇ ;25 were CRF01-AE;2 cases were C and CRF02- AG; lease was CRF03-AB and CRF02-AG. Conclusion HIV-1 subtypes in the blood donors and blood recipients and in drug users were not very different. Sexually transmitted HIV-1 subtype was distributed widely.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2009年第8期1023-1024,共2页
Chinese Journal of Public Health
