摘要
目的研究氯化两面针碱对S180荷瘤小鼠的的抗肿瘤作用及机制。方法以荷瘤小鼠实体瘤瘤重为实验指标评价氯化两面针碱对S180移植性肉瘤的抑制作用,免疫组织化学方法检测肿瘤组织中的p53、Bcl-2和Bax蛋白。结果氯化两面针碱2.5,5和10 mg.kg-1剂量对S180肉瘤生长的抑制率分别为1.95%,27.3%和42.9%,p53、bcl-2蛋白的表达率明显低于对照组,bax蛋白表达率高于对照组。结论氯化两面针碱对小鼠S180肉瘤有抑制作用,其机制可能与影响癌基因的表达有关。
Objective To investigate the anti - tumor effect of Nitidine chloride in vivo on Sarcoma180 （ S180 ） mice and its mechanism. Methods The antitumor effect of Nitidine chloride was evaluated by tumor weight. The expressions of p53,bcl -2 and bax proteins in the tissue were studied by immunohistochemistry. Results Nitidine chloride significantly inhibited the growth of tumor, the inhibitory rate at the dose of 2.5,5,10mg · kg^-1 was 1.95% ,27.3% and 42.9% respectively. The expression of p53 ,bcl - 2 proteins was decreased obviously in the Nitidine chloride group as compared to control group, and the expression of bax was increased. Conclusion Nitidine chloride has significant antitumor effect in vivo, and its influence on oncogene may be one of its anti - tumor mechanism.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2009年第6期1389-1390,共2页
Lishizhen Medicine and Materia Medica Research
基金
广西科学研究与技术开发计划项目(No:桂科攻0630002-2E)
广西大型仪器协助共用网经费资助
关键词
两面针
氯化两面针碱
抗肿瘤
中药
Zanthoxylum nitidum （ Roxb. ） DC
Nitidine chloride
Anti - tumor
Traditional Chinese medicine
