摘要
目的观察氯化两面针碱(NC)的抗肿瘤作用。方法采用MTT法观察NC体外对肿瘤细胞存活的影响。移植性S180肉瘤模型小鼠,ip给予NC,每天1次,共10d,测瘤重,计算抑瘤率,并在电镜下观察移植瘤细胞的超微结构。腹水型H22肝癌模型小鼠,sc给予NC,每天1次,共10d,观察小鼠的存活时间,计算生命延长率。结果体外给予NC0.625,1.25,2.5,5.0和10.0mg·L-1可浓度依赖性地降低人鼻咽癌细胞CNE1、人肺癌细胞SPC-A-1和人乳腺癌细胞MDA-MB-231等9种肿瘤细胞的存活率,作用48h平均IC50为(3.33±0.28)mg·L-1。体内给予NC2.5,5.0和10.0mg·kg-1对小鼠S180肉瘤的抑瘤率分别为1.95%,27.3%和42.9%,对腹水型H22肝癌小鼠的生命延长率分别为35.7%,71.4%和85.7%。电镜下可见NC10.0mg·kg-1组S180移植瘤细胞核染色质浓缩并边缘化,核碎裂,胞浆空泡化明显。结论NC体内外应用均具有明显的抗肿瘤作用。
AIM To investigate the antitumor effect of nitidine chloride （NC） in vitro and in vivo. METHODS MTT assay was used to ex- amine the survival of tumor cells in vitro. The implanted sarcoma 180 （S180） model mice were ip given NC, once daily for 10 d, then the tumor weights were measured and the tumor in- hibition rates were calculated. The ultrastruc- ture of implanted S180 cells was observed with electron microscope. The ascitic-tumor model mice induced by hepatocarcinoma H22 were sc given NC, once daily for l0 d, then mouse life span was recorded, and the life prolonged rates were calculated. RESULTS In vitro, NC 0.625, 1.25, 2.5, 5.0 and 10.0 mg·L-1 decreased survival rates of tumor cells in a con- centration-dependent manner, and the average of IC50 value for 48 h was （3.33 ± 0.28） mg·L-1. In vivo, NC 2.5, 5.0 and 10.0 mg·L-1 inhibited the growth of S180 tumor and tumor inhibition rates were 42.9%, respectively. 1. 95%, 27. 3% and NC significantly pro- longed survival time of H22 ascitic-tumor mice, and life prolongation rate was 35.7%, 71.4% and 85. 7% , respectively. Condensation of chromatin at margins of nuclei, disintegration of nucleoli and vacuoles in cytoplasm were observed in NC 10.0 mg-kg- 1 group with electron microscope. CONCLUSION NC has signifi- cant antitumor effect in vitro and in vivo.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2009年第3期214-218,共5页
Chinese Journal of Pharmacology and Toxicology
基金
国家自然科学基金资助项目(30760302)
国家自然科学基金资助项目(30850010)
广西科学研究与技术开发计划资助项目(0630002-2E)~~
