摘要
以百里酚作显色剂，用分光光度法测定烟叶中氨的显色条件［１］。在氯胺Ｔ存在和碱性条件下，百里酚和氨生成稳定的水溶性有色化合物。该化合物在６７０ｎｍ处有最大吸收峰，体系中氨的浓度在０～６μｇ／ｍｌ范围内符合比耳定律。用于测定烟叶中的氨，回收率为９６％～１００％，相对标准偏差小于０．３０％。
A spectrophotometric method is presented for the determination of ammonia in tobacco. Determination of ammonia based on the reaction with Chloramine-T and Thymol to give a color compound in an alkaline solution. The maxium absorbance of the compound is at 670nm, and Beer′s law is obeyed in the range of 0～6μg/ml. Ammonia in tobacco were determined by the proposed method, and its recovery rate is 96%～100%, relative standard deviation is less than 0.30%.
出处
《烟草科技》
EI
CAS
1998年第3期21-23,共3页
Tobacco Science & Technology
关键词
百里酚
分光光度法
烟叶
氨
Thymol Spectrophotometry Tobacco\ Ammonia
