摘要
【目的】阐明微卫星座位OarJL36与小尾寒羊高繁殖力主效基因FecB之间的连锁关系,为绵羊高繁殖力的标记辅助选择提供依据。【方法】分析与FecB基因最紧密连锁的微卫星座位OarJL36在高繁殖力绵羊品种(小尾寒羊和湖羊)与低繁殖力绵羊品种(特克塞尔和多赛特)中的遗传多态性,探讨该微卫星与小尾寒羊FecB基因的连锁不平衡关系。【结果】高繁殖力小尾寒羊和湖羊在骨形态发生蛋白受体IB(bone morphogenetic protein receptor IB,BMPR-IB)基因编码序列第746位碱基处发生了与Booroola Merino绵羊相同的FecB突变(A746G),低繁殖力特克塞尔和多赛特绵羊则未发生这种突变;小尾寒羊BB、B+、++型频率分别为0.500、0.386和0.114,湖羊BB、B+、++型频率分别为0.800、0.200和0.000;微卫星座位OarJL36在4个绵羊品种463个个体中共检测到10个等位基因和31种基因型,最小等位基因为160bp,最大等位基因为200bp;182bp等位基因在小尾寒羊(n=308)和湖羊(n=60)中处于优势等位基因,频率分别为0.696和0.925,在特克塞尔(n=47)和多赛特绵羊(n=48)中的频率分别为0.085和0.125,在BB型小尾寒羊(n=154)和BB型湖羊(n=48)中该等位基因频率更高,分别为0.916和0.938,而在++型小尾寒羊(n=35)中的频率为0.129;OarJL36在小尾寒羊、湖羊、特克塞尔、多赛特以及BB、B+、++型小尾寒羊和BB、B+型湖羊中的多态信息含量分别为0.475、0.139、0.661、0.768、0.152、0.588、0.843、0.115和0.212;连锁不平衡分析显示小尾寒羊FecB基因B等位基因与OarJL36微卫星座位182bp等位基因之间存在较强的连锁关系,但仍有一定的重组(D′=0.614),而+等位基因与OarJL36微卫星座位上的160、192、196、200bp等位基因完全连锁(D′=1.000)。【结论】OarJL36微卫星座位182bp等位基因与小尾寒羊FecB基因B等位基因之间存在较强的连锁关系,是与小尾寒羊多羔主效基因紧密连锁的一个遗传标记。
[Objective] The objectives of the present study were to elucidate the linkage relationship between microsatellite locus OarJL36 and FecB gene of Small Tail Han sheep and to provide a scientific basis for marker-assisted selection for high prolificacy in sheep. [Method] Genetic polymorphisms ofmicrosatellite locus OarJL36 which was the closest linked marker to the ovine prolificacy major gene FecB were detected in high fecundity sheep breeds （Small Tail Han and Hu sheep） and low fecundity sheep breeds （Texel and Dorset）. The linkage disequilibrium between microsatellite locus OarJL36 and FecB gene of Small Tail Han sheep was also analyzed. [Result] There was a same FecB mutation （A746G） ofBMPR-1B gene in both Small Tail Han and Hu sheep as that in Booroola Merino ewes. The same mutation did not exist in both Texel and Dorset sheep. The fxequency of BB, B＋and ＋＋ genotype was 0.500, 0.386 and 0.114 in Small Tail Han sheep, and was 0.800, 0.200 and 0.000 in Hu sheep, respectively. Ten alleles and 31 genotypes were detected at OarJL36 in 463 individuals from four sheep breeds, in which 160 bp was the shortest allele and 200 bp was the longest allele. The 182 bp was the predominant allele in Small Tail Han （n=308） and Hu sheep （n=60）, of which frequency was 0.696 and 0.925, but 0.085 and 0.125 in Texel （n=47） and Dorset （n=48）. Especially, the 182 bp allele frequency was 0.915 and 0.938 in BB genotype group from Small Tail Han and Hu sheep, but 0.129 in ＋＋ genotype group from Small Tail Han sheep. The polymorphism information content （PIC） was 0.475, 0.139, 0.661, 0.768, 0.153, 0.588, 0.843, 0.116 and 0.212 in Small Tail Han, Hu, Texel, Dorset, BB group from Small Tail Han, B＋ group from Small Tail Han, ＋＋ group from Small Tail Han, BB group from Hu sheep, B＋ group from Hu sheep, respectively. In Small Tail Han sheep, linkage analysis indicated that there were fervent linkage disequilibrium as well as certain recombination between 182 bp allele of microsatellite locus OarJL36 and B allele of FecB gene （D＇=0.614）, and complete linkage between 160 bp,192 bp, 196 bp, 200 bp alleles of microsatellite OarJL36 and ＋ allele ofFecB gene （D＇=1.000）. [Conclusion] These results preliminarily showed that 182 bp allele ofmicrosatellite locus OarJL36 is a genetic marker closely linked to B allele of the prolificacy major gene FecB in Small Tail Han sheep.
出处
《中国农业科学》
CAS
CSCD
北大核心
2009年第6期2133-2141,共9页
Scientia Agricultura Sinica
基金
国家科技基础条件平台建设计划(2005DKA21101)
国家现代农业产业技术体系建设专项资金(农科教发[2008]10号)
国家“973”计划(2006CB102105)
国家“863”计划(2002AA211081、2005AA211080)
国家科技支撑计划(2008BADB2B01、2006BAD01A11、2006BAD13B08)
国家自然科学基金项目(30140004、30300248)
北京市科委科技计划(D07050550000701、Y0705003041131)
