摘要
利用“forced”限制性酶切片段长度多态性PCR技术 ,检测湖羊、小尾寒羊和新疆细毛羊的DNA样本是否存在Booroola (FecB)基因。结果表明 ,12头湖羊均为纯合型Booroola基因携带者 ;12头小尾寒羊中 ,4头为Booroola基因纯合型 ,7头为杂合型 ,1头不携带Booroola基因 ;12头新疆细毛羊均不携带Booroola基因。
Thirty six DNA samples from Hu and Han (small tail) and Xinjiang fine wool ewes were prepared to detect the Booroola(FecB)gene by forced restriction fragment length polymorphism PCR (FRFLP PCR).The results showed that the Booroola gene is present in both Hu and Han sheep: All twelve Hu ewes were homozygous,four of Han ewes were homozygous,seven were heterozygous and one was none carrier for FecB .All twelve Xinjiang sheep tested non carrier for FecB .
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2003年第1期104-106,共3页
Journal of Nanjing Agricultural University
基金
中国新西兰合作项目
