摘要
目的在临床微生物实验室建立简便的纸片扩散试验方法,用于产金属β-内酰胺酶的铜绿假单胞菌的检测。方法以头孢他啶、亚胺培南为底物,乙二胺四乙酸二钠(EDTA-Na2)和2-巯基乙醇(2-ME)为酶抑制剂,在MH平板上用纸片扩散法进行抗生素协同敏感试验,并与聚合酶链反应(PCR)检测金属β-内酰胺酶基因结果进行比较。结果任一药敏纸片与含酶抑制剂纸片间出现抑菌环扩大现象则表示受试菌产金属β-内酰胺酶表型初筛试验阳性,在144株受试菌中,协同试验阳性29株,PCR检测金属β-内酰胺酶阳性26株。结论CAZ-EDTA纸片协同法在检测产IMP-1型金属酶的铜绿假单胞菌时的特异性和敏感性可与PCR技术相媲美,适用于临床微生物实验室对产金属β-内酰胺酶铜绿假单胞菌的日常初步鉴定。
Objective To establish a convenient test for detection metallo-β-lactamase-producing Pseudomonas aeruginesa in the clinical microbiology laboratory.Methods Substrates were ceftazidime,imipenem and enzyme inhibitor were 2-memaptoethanol and EDTA-Na2.The microbiology sensitivity synergic tests were processed by K-B method in the M-H agar and compared with the PCR results of metallo-beta-lactamase gene detection. Results The metallo-beta-lactamase-producing aeruginosasynergized with EDTA-Na2 disc at least in one of the three kinds of substrates. In 144 imipenem and ceftazidime resistant strains, the metallo-beta-lactamase positive strains were detected by this method was 26, and that by PCR was 29. Conclusion The specificity and sensitivity of CAZ-EDTA Synergic Test were comparable to those of PCR. Therefore, this convenient test would be valuable for daily use in clinical laboratories.
出处
《中国实验诊断学》
北大核心
2009年第5期578-581,共4页
Chinese Journal of Laboratory Diagnosis
