摘要
目的:检测并分析Ezrin蛋白和c-Met蛋白在正常胃粘膜、肠上皮化生、不典型增生及胃癌组织中表达的差异性和关联性,探讨Ezrin蛋白及c-Met蛋白的表达与胃癌发生和转移的关系及意义。方法:收集手术切除胃癌标本219例(其中188例同时于距癌灶边缘大于5cm处取配对正常胃粘膜作为对照)、不典型增生75例及肠上皮化生85例。采用组织芯片制作仪构建胃癌及其癌前病变组织芯片,利用免疫组化方法检测Ezrin蛋白和c-Met蛋白在正常胃粘膜、肠上皮化生、不典型增生及胃癌中的表达。结果:除去抗原修复造成的部分组织脱落,最终可用病例分别为正常胃粘膜131例,肠上皮化生55例,不典型增生62例,胃癌182例。Ezrin蛋白在肠上皮化生(94.55%)、不典型增生(87.10%)和胃癌(83.52%)中表达的阳性率显著高于正常胃粘膜(61.07%),P<0.01;伴淋巴结转移胃癌组Ezrin蛋白表达的阳性率(88.32%)显著高于无淋巴结转移组(68.89%),P<0.01。c-Met蛋白在肠上皮化生(63.64%)、不典型增生(61.29%)和胃癌(65.93%)中表达的阳性率显著高于正常胃粘膜(28.24%),P<0.01;伴淋巴结转移胃癌组c-Met蛋白表达的阳性率(71.53%)显著高于无淋巴结转移组(48.89%),P<0.05。结论:Ezrin蛋白可能参与胃粘膜肠上皮化生和肠型胃癌的发生;Ezrin蛋白和c-Me蛋白共同参与了胃癌的发生和发展,但其具体分子机制尚需进一步深入研究。
Objective： To detect the expression of Ezrin protein and c-Met protein in normal mucosa, intestinal metaplasia, atypical hyperplasia and gastric cancer, and to explore its correlation with gastric carcinogenesis and metastasis. Methods： Surgically resected specimens from 219 cases of gastric cancer （188 samples of normal gastric mucosa at greater than 5cm from the edge of carcinoma were taken as control） and 75 cases of atypical hyperplasia and 85 cases of intestinal metaplasia were collected. Tissue microarray including gastric cancer and precancerous lesions were constructed. Immunohistochemistry was used to detect the expression of Ezrin protein and c-Met protein in normal gastric mucosa, intestinal metaplasia, atypical hyperplasia and gastric cancer. Results： Because of the shedding of some tissues caused by antigen retrieval, finally there were 131 cases of normal gastric mucosa, 55 cases of intestinal metaplasia, 62 cases of atypical hyperplasia, and 182 cases of gastric cancer. The positive rates of Ezrin protein in intestinal metaplasia （94.55%）, atypical hyperplasia （87.10%） and gastric cancer （83.52%） were significantly higher than that in normal gastric mucosa （61.07%）. In gastric cancer with lymph node metastasis, the positive rate of Ezrin protein （88.32%） was significantly higher than that in the group without lymph node metastasis （68.89%） （P〈0.01）. The positive rate of c-Met protein in intestinal metaplasia （63.64%）, atypical hyperplasia （61.29%） and gastric cancer （65.93%） were significantly higher than that in normal gastric mucosa （28.24%） （P〈0.01）. In gastric cancer with lymph node metastasis, the positive rate of c-Met protein （71.53%） was significantly higher than that in the group without lymph node metastasis （48.89%） （P〈0.05）. Ccnclusicn： Ezrin protein may be involved in the occurrence of intestinal metaplasia and gastric cancer of intestinal type. Ezrin protein and c-Met protein may be involved in the occurrence and progression of gastric cancer. Further study is needed to explore the corresponding molecular mechanism.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2009年第7期404-408,共5页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金(编号:30371607)
辽宁省高等学校优秀人才支持计划基金资助(编号:2006124)~~
