摘要
本文选用100对SSR引物对12个抗感黄曲霉花生品种的基因组DNA进行扩增,结果表明共有41对引物在不同品种间检测出2～4个等位基因,多态性信息量(PIC)为0.153～0.750。供试品种SSR标记基因型与黄曲霉侵染指数间的关联分析表明有5个标记与抗性相关,其中标记pPGSseq19D9与抗性关联度最高,Pearson相关系数达0.913。进一步观察发现pPGSseq19D9的扩增带型能直接区分抗感品种,初步推断pPGSseq19D9可能与一个贡献率较大的抗黄曲霉基因连锁。
100 pairs of SSR were used to amplify the genomic DNA of 12 resistant andsusceptible peanut varieties infected by Aspergillus flavus. The results showed that polymorphisms were detected only by 41 SSR primer pairs, with 2～4 alleles per locus in these varieties. The value ofPIC （polymorphism information content） varied from 0.153 to 0.750. Correlation analysis of SSR markers and host resistance to A spergiUus flavus infection in peanut （A rachis hypogaea L.） showed that 5 markers were found to have a correlation with host resistance, and Pearson＇s correlation coefficient reached to 0.913. A marker named pPGSseq19D9 showed the highest correlation value among them. Upon further investigation, we found that the band amplified by pPGSseq19D9 can differentiate the resistant and susceptible varieties. A preliminary deductions was made that pPGSseq19D9 may be linked with one major gene which controls the resistance to A spergillus flavus infection.
出处
《分子植物育种》
CAS
CSCD
2009年第2期360-364,共5页
Molecular Plant Breeding
基金
国家863项目(2006AA10Z156
2006AA100106)
广东省自然科学基金项目(06025389
07117967)
广东省农业攻关项目(2006A20201003)资助
关键词
花生
SSR
黄曲霉
关联分析
Peanut （Arachis hypogaea L.）, SSR, Aspergillus flavus, Correlation analysis
