摘要
通过组织块培养法进行胎鼠原代细胞培养,采用刮除法和相差消化及相差贴壁法对细胞进行纯化,用0.05%的胰蛋白酶进行消化传代对小鼠小肠上皮细胞进行了原代分离培养、传代、纯化及鉴定。结果表明:组织块培养法得到了小鼠生长状况良好的细胞,经过纯化,得到了较纯的小肠上皮细胞;形态学观察和免疫组化检测显示得到的细胞为小鼠上皮细胞。用组织块培养可以分离纯化出增殖能力较强的上皮细胞,并能进行传代纯化,通过免疫组化鉴定,证明分离出的小鼠IEC为阳性,即为小肠上皮细胞。
The purpose of this experiment was to study the way which could separate, passage and purification of mouse intestine epithelium. Through tissue culture method, this test focused on primary cell culture, using curettage law, difference digest and adherent law to purify cells, with 0.05 percent of try sin to digest passage. The results showed that.by tissue culture, the growth of ceils in good condition could be got, after purification, more pure mouse intestinal cells had been got. The cells which could be showed from morphology observation and immunization section test was mice cells. Tissue culture could differentiate and purify epithelial cells which had higher proliferation ability, and conduct passage purified; through immunohistochemical identification, the mice IEC isolated from was positive, that are epithelial cells.
出处
《家畜生态学报》
2009年第1期48-51,共4页
Journal of Domestic Animal Ecology
基金
江苏省高等学校大学生实践创新项目(编号:462)
国家自然基金(编号:30471259)
关键词
小鼠
上皮细胞
原代培养
鉴定
mice
epithelial cells
primary culture
identification
