摘要
为了研究二系杂交小麦HMW-GS类型与蛋白质含量、沉淀值、面团形成时间和衰弱角斜率等品质性状的关系,采用SDS-PAGE方法分析了杂交种的高分子量麦谷蛋白亚基(HMW-GS)的组成。结果表明,在试验材料中普遍存在有劣质亚基N和2+12,它们的频率分别是60.4%,83.0%。在HMW-GS与F1籽粒品质性状关系的研究中发现,各位点对蛋白质含量的贡献率是Glu-B1>Glu-A1>Glu-D1;对沉淀值贡献率是Glu-B1>Glu-D1>Glu-A1;对形成时间和衰落角斜率的贡献率是Glu-D1>Glu-B1>Glu-A1。总体来说,位点按对品质的贡献率大小依次为Glu-B1>Glu-D1>Glu-A1。就单个亚基而言Glu-D1位点,杂合2+12/5+10>纯合2+12;Glu-A1位点蛋白质含量、SDS沉淀值和形成时间均为N/N>N/1;在Glu-B1位点,蛋白质含量是7+8/17+18=7+9/17+18=7+8/7+9=7+8/7+8>7+9/7+9;沉淀值的大小依次为7+8/17+18≥7+9/17+18=7+8/7+9=7+8/7+8≥7+9/7+9;形成时间是7+8/17+18>7+9/17+18=7+8/7+9=7+8/7+8=7+9/7+9;衰弱角斜率大小是7+9/17+18≥7+8/17+18=7+9/7+9≥7+8/7+9=7+8/7+8。在不同亚基组合中,具有N/N,7+8/7+8,2+12/5+10亚基的品质最好。杂合位点5+10亚基对2+12亚基的品质具有补偿效应。
In order to establish the relationships between high molecular weight glutenin subunits （HMW-GS）type and the flour quality parameters of protein content, sedimentation volume, dough development time and feeble angle slope in two-line system hybrid wheat, the SDS-PAGE was used to analyze the compositions of HMW-GS of hybrid wheat seeds. The result indicated that the inferior subunits N and 2 ＋ 12 existed universally in the experimental materials. Their frequency were 60.4 % and 83.0 %, respectively. The relationships between HMW-GS type and the flour quality characters of Fl were ranked as follows：in protein content Glu-B1 〉 Glu-A1 〉 Glu-D1 ;in sedimentation volume Glu-B1 〉 Glu-D1 〉 Glu-A1 ; in dough development time and feeble angle slope Glu-D1 〉 Gtu-B1 〉 Glu-A1, respectively. In all, the order of contributions to the flour quality character was Glu-B1 〉 Glu-D1 〉 Glu-A1. As far as individually subunit was concerned, at Glu-D1, the heterogeneous 2 ＋ 12/5 ＋ 10 subunits was higher than homogeneous 2 ＋ 12 subunits;at Glu-A1, the order in sedimentation volume, protein content and dough development time was N/N 〉 N/1 ; at Glu-B1, in protein content,it was ranked as 7 ＋ 8/17 ＋ 18 = 7 ＋ 9/17 ＋ 18 = 7 ＋ 8/7 ＋ 9 = 7 ＋ 8/7 ＋ 8 〉 7 ＋ 9/7 ＋ 9;in sedimentation vol- ume,7 ＋ 8/17 ＋ 181〉7 ＋ 9/17 ＋ 18 = 7 ＋ 8/7 ＋ 9 = 7 ＋ 8/7 ＋ 81〉7 ＋ 9/7 ＋ 9;in dough development time,7 ＋ 8/17 ＋ 18 〉7＋9/17＋18=7＋8/7＋9=7＋8/7＋8=7＋9/7＋9;infeebleangleslopewas7＋9/17＋181〉7＋8/17＋18=7 ＋ 9/7 ＋ 91〉7 ＋ 8/7 ＋ 9 = 7 ＋ 8/7 ＋ 8.As a whole, HMW-GS combination N/N,7 ＋ 8/7 ＋ 8,2 ＋ 12/5 ＋ 10 was the best quality in all of the hybrid wheat. This can be ascribed as the compensate effects of the good quality HMW-GS 5 ＋ 10 on the worse ones 2 ＋ 12 in heterozygous condition.
出处
《华北农学报》
CSCD
北大核心
2009年第1期149-153,共5页
Acta Agriculturae Boreali-Sinica
基金
国家高技术研究发展计划(“863”计划)项目(2006AA100102)
国家科技支撑计划项目(2006BAD01A02)
北京农业育种基础研究创新平台项目
北京市自然科学基金项目(5082005)
北京市农林科学院青年科研基金项目
