摘要
目的：研究半边旗抗肿瘤有效成分６Ｆ对ＨＬ－６０细胞周期的影响及对常用抗肿瘤药的体外增效作用。方法：应用流式细胞光度术（ＦＣＭ）测定细胞周期，应用噻唑蓝（ＭＴＴ）法测定药物对细胞的抑制率。结果：不同浓度６Ｆ作用６小时即可使ＨＬ－６０细胞Ｓ期及Ｇ２／Ｍ期比例升高，Ｇ１期比例下降，并呈一定的剂量效应关系，当作用到２４小时后，Ｓ期比例进一步升高，但Ｇ２／Ｍ期比例稍有回落。低浓度６Ｆ分别与２－氯代脱氧腺苷（２－ＣＬｄＡｄｏ），顺铂（ＣＤＤＰ），长春新碱（ＶＣＲ），氟尿嘧啶（５ＦＵ）合用可增强它们对ＨＬ－６０细胞的杀伤作用，ｑ值大于０．８５，与各药有相加或协同作用，６Ｆ对２－ＣｌｄＡｄｏ，ＣＤＤＰ，ＶＣＲ，５ＦＵ的增效倍数分别为１．５８，１．５３，１．５５，１．３８。结论：６Ｆ可明显阻断ＨＬ－６０细胞在Ｓ期及Ｇ２／Ｍ期；６Ｆ可增强上述药物对ＨＬ－６０细胞的杀伤作用。已知，２－ＣｌｄＡｄｏ阻断细胞在Ｓ期，ＣＤＤＰ和ＶＣＲ阻断Ｇ２／Ｍ期，５ＦＵ阻断Ｇ１期。鉴于所试药物对细胞周期的影响不同，提示６Ｆ的体外增效作用可能与此有关。
Objective: To observe the effect of 6F, one of the diterpenoids isolated from Pteris semipinnata L. (PsL), on the HL-60 cell cycle kinetics and its enhanced cytotoxicity when combined with other antitumor agents. Methods: Cell cycle was determined using F1ow Cytometry (FCM) analysis, Cytotoxicity assay was tested by MTT method. Results: The FCM data showed that the distribution of HL-60 cells among phases of the cell cycle was changed when treated with 6F. After exposure of the cells to various concentration of 6F for 6h, the S and G2/M phase ratio was increased significantly in a dose-dependent manner. After 24h of exposure, S hpase ratio increased further. The inhibitory rates of 6F combined with 2-CldAdo or CDDP or VCR or 5FU were higher than that of these drugs used alone, q values were more than 0.85. Their enhancement times by 6F were 1.58, 1.53, 1.55, 1.38, respectively. Conclusion: The results indicated that 6F could arrest HL-60 cells in S and G2/M phase, 6F could enhanced the cytotoxicity of the drugs mentioned above. it is known that 2-CldAdo arrests cells in S phase, CDDP and VCR arrest cells in G2/M phase, 5FU arrests cells in G1 phase. Thr different effect of these agents on the various phases of cell cycle kinetics may be partially explained the enhanced effect of 6F.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1998年第3期191-193,共3页
Chinese Journal of Cancer
基金
广东省高教局重点学科专款资助
