摘要
考察了培养基的配比、部分简单碳源对黑曲霉AS3.350产糖化酶、蛋白酶和孢子收获量的影响.结果表明:在选择的2种有机氮源中,豆粕可被黑曲霉较好地利用.在麸皮与豆粕的质量比为5∶5,30℃培养72 h时,糖化酶活力最高为1 185 U/g,蛋白酶活力差异不显著.在分别添加葡萄糖0.5%,蔗糖0.75%时,黑曲霉产糖化酶酶活力达到最高;对添加淀粉来说,随着淀粉浓度的增高而糖化酶活力逐渐增大.蛋白酶活力和孢子收获量与碳源的种类、浓度以及菌体的生长情况密切相关.
The effects of some medium components, including culture medium proportions and several simple carbon sources, on protease and glucoamylase activity of aspergillus niger were investigated. The results show that among two kinds of organic nitrogen sources soybean meal could be used efficiently by aspergillus niger, the optimal conditions of culture medium for enzyme secretion was the mass ratio of wheat bran and soybean meal of 5 ： 5 and the glucoamylase activity of 1 185 U/g, when the cultivating condition was 20 g medium in 250 mL flask and incubation temperature of 30 ℃ for 72 hours. The protease activity had no obvious difference among the three samples. The glucoamylase activity was most when the glucose of 0. 5 % and sucrose of 0. 75 % were added in the basic medium respectively. The glucoamylase activity increased with the increasing of starch concentration. The protease activity and spore yield were closely correlated with the kind, concentration of carbon sources and growth of aspergillus niger.
出处
《中国矿业大学学报》
EI
CAS
CSCD
北大核心
2009年第1期144-148,共5页
Journal of China University of Mining & Technology
基金
国家高技术研究发展计划(863)项目(2006AA102314)
徐州市社会发展基金项目(xz06063)
关键词
黑曲霉
糖化酶
蛋白酶
营养成分
aspergillus niger
glucoamylase
protease
nutrients
