摘要
目的观察不同时期,不同转移阶段的人乳腺癌组织中细胞间黏附分子(ICAM-1)和淋巴细胞功能相关抗原1(LFA-1)的表达,探讨ICAM-1和LFA-1对乳腺癌淋巴道转移的影响,为乳腺癌淋巴道转移的分子机制提供理论依据。方法根据不同病变阶段的手术患者切除的乳腺癌标本进行HE染色和免疫组化染色,观察ICAM-1、LFA-1在乳腺癌组织和ICAM-1、LFA-1、VEGFR-3在癌旁组织淋巴管内皮的表达情况。结果随着临床分期的增高和淋巴结转移的发生,ICAM-1和LFA-1在乳腺癌癌细胞和癌巢中的表达阳性率逐渐增高,同时ICAM-1、LFA-1和VEGFR-3在癌旁组织淋巴管内皮细胞的表达阳性率也逐渐增高,具有正相关性,并且ICAM-1和LFA-1在癌旁组织淋巴管内皮细胞的表达之间具有正相关性(P<0.01)。同时发现无论是在乳腺癌癌细胞、癌巢还是在癌旁组织淋巴管内皮细胞,ICAM-1和LFA-1表达的高低都与癌的分化程度、性别、年龄不具有相关性,差异无统计学意义(P>0.05)。结论ICAM-1和LFA-1与乳腺癌的淋巴道转移有关,并且通过两者的协同作用促进了乳腺癌的淋巴道转移。两者在癌组织及癌旁组织淋巴管内皮细胞表达的高低,可以作为数据指标对乳腺癌淋巴道转移的预防和治疗起到一定的指导作用。随着肿瘤的进展,乳腺癌癌旁组织中的淋巴管数量随之增加,可能与乳腺癌的淋巴道转移有关。VEGFR-3在淋巴管内皮细胞的表达具有较高的特异性,可以作为淋巴管内皮细胞的特异性标志物来标志淋巴管。
Objective To explore the influence of ICAM - 1 and LFA - 1 on the breast cancer metastasis and provide scientific base for studying the molecular mechanism of breast cancer metastasis by observing the expression of ICAM - 1 （ intercellular adhension moleculel ） and LFA - 1 （Lymphocyte function related antigen 1 ） in different time different metastasis stage of breast cancer. Methods We observed the expression of ICAM - 1 and LFA - 1 in breast cancer tissue as well as the expression of ICAM - 1 ,LFA - 1 and VEGFR -3 in Lymphatic vessel endothelial cell of nearby the breast cancer tissues from the patients of the operation in the defferent stages with the methods of HE staining and immunohistochemistry staining. Results The positive rate of 1CAM - 1 and LFA - 1 expression in cancer cell and cancer nest of breast cancer was increased gradually along with the increase of clinical stages and the occurrence of lymph node metas- tasis. And the positive rate of ICAM - 1, LFA - 1 and VEGFR - 3 expression in lymphatic vessel endothelial cell of nearby the breast cancer tissues was increased gradually too along with the increase of clinical stages and the occurrence of lymph node metastasis, which has the positive correlation, and it has the positive correlation that the ICAM - 1 and LFA - 1 expressed in Lymphatic vessel endothelial cell of nearby the breast cancer tissues（ P 〈 0.0l ）. At the same time we discovered that the ICAM - 1 and LFA - 1 expression increase or decrease didnot has the relevance with the cancer split up degree, the sex, the age regardless of in cancer cell and cancer nest of breast cancer or in lymphatic vessel endothelial cell of nearby the breast cancer tissues, and the difference does not have statistics significance （P 〉 O. 05）. Conclusion The ICAM - 1 and LFA - 1 were related with breast cancer lymph metastasis,and the ICAM - 1 and LFA - 1 promo- ted breast cancer lymph metastasis through synergism each other. The increase or decrease of ICAM - 1 and LFA - 1 expression in the breast cancer tissue and lymphatic vessel endothelial cell of nearby the breast cancer tissues could as the data target to display certain instruction function for prevention and treatment of breast cancer lymph metastasis. The Lymphatic vessel quantity of nearby the breast cancer tissues was increased along with tumor progress, which may be related with breast cancer lymph metastasis. VEGFR - 3 has the high specificity in the lymphatic vessel endothelial cell expression, and may act as the lymphatic vessel as the lymphatic vessel endothelial cell specific marker.
出处
《医学研究杂志》
2009年第1期75-79,共5页
Journal of Medical Research
