摘要
本实验研究了苯酚-硫酸法测定杏鲍菇多糖含量时各因素对结果的影响,并对这些因素进行了优化。通过实验发现,波长为485nm、苯酚用量1.4ml、反应温度40℃、反应时间15min,在10.00~70.00μg/ml范围内吸光度与被测物含量呈良好的线性关系,相关系数r为0.9996,平均回收率99.2%,相对标准偏差(RSD)为1.16%。
The factors affacfing the determination of polysaccharide content in Pleurotus eryngii with phenol-sulfuric acid method were optimized. Results showed that under the conditions of detection wavelength 485 nm, phenol content 1.4 ml, reaction temperature 40 ℃ and reaction time 15 rain, there is good linearity range from 10.00 to 70.00 μg/ml (r=0.9996). The average recovery is 99.2% and the RSD is 1.16%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第12期555-558,共4页
Food Science
基金
内蒙古科技攻关项目(20030603)
关键词
杏鲍菇
多糖
苯酚-硫酸法
Peurotus eryngii
polysaccharide
phenol-sulfuric acid method