摘要
为探讨血管紧张素转化酶基因插入／缺失多态性与冠心病的关系，用多聚酶链反应方法检测79例冠心病患者和68例健康人血管紧张素转化酶基因第16内含子中长度为287bP碱基片段的插入／缺入情况、按其存在与否将研究对象分为缺失型纯合子、插入型纯合子和杂合子，同时检测血清血管紧张素转化酶活性。结果发现冠心病组中缺失型基因频率显著高于对照组（P＜0．05），缺失型等位基因频率较对照组亦明显增高（P＜0．05）。对照组与冠心病组间血清血管紧张素转化酶活性比较无明显差别，但两组内不同基因型之间血管紧张素转化酶活性均存在显著差别，缺失型最高，插入型最低。以上结果提示，血管紧张素转化酶基因插入／缺失多态性与冠心病有关，缺失型可能是冠心病发生的危险因素之一，血管紧张素转化酶基因缺失型者冠心病的发生可能与其较高的血清转化酶水平有关。
Aim To study the association between insertion/deletion polymorphism of angiotensin converting enzyme gene and coronary artery disease.Methods The insertion/deletion of a 287 base pairs segment in 16 intron of angiotensin converting enzyme gene in 79 coronary artery disease patients and 68 healthy subjects was detected by polymerase chain reaction. The subjects were divided into three groups:homozygotes for the deletion, homozygotes for the insertion and heterozygotes, according to its presence orabsence. The serum angiotensin converting enzyme activity was also detected.Results The deletion genotype frequency in coronary artery disease group was marked higher than in controls (P<0.05). The frequency of deletion allele was also greater than that in controls (P<0. 05).The serum angiotensin converting enzyrne activity between coronary artery disease patients and controls was similar. But it was significantly different among different genotypes in two groups. The deletion genotype had the highest, insertion genotype had the lowest.Conclusion The insertion/deletion polymorphism of angiotensin converting enzyme gene is associated with coronary artery disease, and the deletion genotype perhaps is a risk factor for coronary artery diseaes. The elevated serum angiotensin converting enzyme level may contribute to the generation of coronary artery disease in patients who have deletion genotype.
出处
《中国动脉硬化杂志》
CAS
CSCD
1997年第4期300-303,共4页
Chinese Journal of Arteriosclerosis
基金
福建省卫生厅科研资助!96011
关键词
ACE
血管紧张素
转化酶
基因多态性
冠心病
Angiotensin converting enzyme
Gene polymorphism
Coronary artery disease
