摘要
用培养基发芽法研究沙芥花粉萌发特性;用人工异株授粉法和联苯胺—过氧化氢法测定柱头可授期。结果表明:沙芥花粉萌发的最佳培养基是20%蔗糖+0.001%硼酸+0.5%琼脂;花粉能够萌发的温度范围为15～38℃,最适萌发温度为25℃;从花蕾充分膨大期至开花后16h花粉活力较强,开花后48h花粉基本无活力;花粉储藏的适宜条件为低温(4℃),储藏期为7d。柱头可授期为开花前48h至开花后96h,开花后24～72h可授性强。
Pollen germination characteristic of Pugionium cornutum （ L. ） Gaertner was evaluated by germination method on culture medium, its stigma receptivity was estimated by the hand cross-pollination and benzidine - H2O2 method. The results showed the optimal pollen germination of P. cornutum was on the culture medium added 20% sucrose, 0. 001% boric acid and 0. 5% agar. The effective temperature of pollen germination was from 15 ℃ to 38 ℃ and the optimal temperature was 25 ℃. The pollen germination rate was very higher from the biggest bud stage to 16 h after blooming, and the pollen viability was disappeared 48 h after blooming. The suitable condition of storing pollen was low temperature （4 ℃ ）, and storing period was 7 d. The stigma of P. cornutum had receptivity from 48 h before blooming to 96 h after blooming, the strong receptivity in 24 -72 h after blooming.
出处
《园艺学报》
CAS
CSCD
北大核心
2008年第10期1473-1478,共6页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(30460080
30260067)
内蒙古科技攻关项目(20050305
20060202)
内蒙古自然科学基金项目(2001108020501
200308020513)
内蒙古人才基金项目
关键词
沙芥
花粉
萌发特性
柱头可授性
温度
培养基
Pugionium cornutum
pollen
germination characteristic
stigma receptivity
temperature
medium
