摘要
光温敏核不育基因在杂种优势利用中具有重大价值,了解其转录和翻译过程对解析温敏核不育机理和分离温敏核不育基因非常重要.利用基因芯片、蛋白质组技术结合遗传分析的方法,以不同温度条件下处理的培矮64S及两优培九自交后得到的F2群体为材料,从转录水平和翻译水平上分别筛选出可能与温敏核不育基因相关的24个特异表达基因以及6个特异表达蛋白质,并分析了这些产物的功能.
The Photo-thermo-sensitive Genic Male-sterile （PTGMS） gene plays an important role in rice heterosis, and it is very important to analyze its expression for elucidating molecular basis of PTGMS and cloning the gene. By microarray and proteomic technology combined with genetic analysis, 24 genes and 6 proteins, expressed specially in PTGMS rice Peiai 64S and F2 population when treated with different temperature, were screened. Their functions were also analyzed by informatics.
出处
《生命科学研究》
CAS
CSCD
2008年第2期126-130,共5页
Life Science Research
基金
国家重点基础研究发展计划973项目(2001CB1088)
关键词
水稻
光温敏核不育基因
基因芯片
蛋白质组
rice
Photo-thermo-sensitive Genic Male-sterile（PTGMS）
gene chip
proteome
