摘要
背景：采用仿生学原理，利用海藻酸钙、纳米羟基磷灰石及胶原按一定比例复合，制成一种可注射、可任意塑形且具有良好骨传导、骨诱导性的组织工程骨，其应用在微创外科技术中，具有组织损伤小、不破坏修复区血供、操作简便易行等优点。目的：验证海藻酸钙纳米羟基磷灰石胶原复合材料与兔骨髓基质干细胞的相容性。设计、时间及地点：对比观察实验，于2008-02／05在南方医科大学珠江医院中心实验室完成。材料：2周龄健康新西兰大白兔用于骨髓基质干细胞的分离培养。海藻酸钙纳米羟基磷灰石胶原复合材料由清华大学材料系研制提供，孔隙率90％，孔径50～200μm。方法：选取生长良好的第5代兔骨髓基质干细胞与海藻酸钙纳米羟基磷灰石胶原复合材料体外复合培养。主要观察指标：共培养5d后倒置显微镜下观察细胞在材料表面、孔隙内的生长情况，扫描电镜下观察细胞在材料上附着情况。细胞和支架共培养后CCK-8法测定细胞活性。结果：骨髓基质干细胞能在海藻酸钙纳米羟基磷灰石胶原复合材料上良好地黏附、增殖、生长，细胞活性未受到海藻酸钙纳米羟基磷灰石胶原复合材料的影响。结论：海藻酸钙纳米羟基磷灰石胶原复合材料与兔骨髓基质干细胞有良好的细胞相容性。
BACKGROUND： The injectable and shapable tissue engineering bone with osteoconduction and osteoinduction nature can be constructed by combining nano-hydroxyapatite, collagen and calcium alginate according to the bionics. It caters for the developmental demand of minimally invasive technology due to many advantages such as small tissue damage, no destroying the blood supply of repaired region, simple and practicable operation. OBJECTIVE： To assess the compatibility of the nano-hydroxyapatite/collagerdcalcium alginate composite and rabbit bone marrow stem cells. DESIGN, TIME AND SETTING： Control observations were carded out from February to May in 2008 in the Central Laboratory of Zhujiang Hospital, Southern Medical University. MATERIALS： Healthy and two-week-old New Zealand rabbits were used to isolate and culture bone marrow stem cells. The nano-hydroxyapatite/collagen/calcium alginate was purchased from Materials Department of Tsinghua University （porosity 90%, pore diameter 50-200 μm）. METHODS： The bone marrow stem cells at the fifth passage were cocultured with nano-hydroxyapatite/collagen/calcium alginate in vitro. MAIN OUTCOME MEASURES： At 5 days, the growth of cells on the surface and pore of materials were observed under inverted microscope; Scanning electron microscope was used to observe the adherence of cells; Cell vitality was detected by using CCK-8 method. RESULTS： The bone marrow stem cells could adhere to, proliferate and grow normally on the nano-hydroxyapatite/ collagen/calcium alginate. The cellular function activity was not affected by the material. CONCLUSION： The nano-hydroxyapatite/collagen/calcium alginate is well biocompatible to rabbit bone marrow stem cells.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第36期7022-7026,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
广东省自然科学基金资助(7005193):可注射可塑形纳米骨修复材料的研制与动物实验研究~~
