摘要
本文采用单一性底物平板分离法和Folin法,观察其水解圈大小和测酶活力,从牛乳中共获得10株产耐高温蛋白酶的嗜冷菌。对菌体形态、染色反应、培养性状、生理生化性状进行了系统研究。鉴定结果表明,上述10个细菌菌株分别属于气单胞菌属(Aeromonas)、巴斯德氏菌属(Pasteurella)、黄杆菌属(Flavobacterium)、变形菌属(Proteus)、节杆菌属(Arthrobacter)、短杆菌属(Brevibacterium)、微球菌属(Micrococcus)、片球菌属(Pediococcus)、乳球菌属(Lactococcus)和明串球菌属(Trichococcus)。
Ten strains of Psychrophilies that producing heatproof protein enzymes were screened out by prate separation and Folin methods Meanwhile, it can also observe their proteclytic circle and proteclytic activity to obtain them. and were identified as Aeromonas, Pasteurella Flavobacterium, Proteus, Arthrobacter, Brevibacterium, Micrococcus, Pediococcus, Lactococcus and Trichococcus based on bacterial modality deying reaction,cultural characteristics, physiological characteristics and biochemical reactions.
出处
《世界科技研究与发展》
CSCD
2008年第4期410-413,共4页
World Sci-Tech R&D
关键词
嗜冷菌
耐高温蛋白酶
分离
鉴定
psychrophilies
heatproof protein enzyme
isolation
identification
