摘要
目的:观察蒙医温针对运动性疲劳大鼠的行为以及下丘脑-垂体-肾上腺皮质(HPA)轴和免疫系统的影响,探讨有关神经-内分泌-免疫机制。方法:选用SD雄性大鼠,分为正常对照组(20只)、模型组(20只)和温针组(19只)。采用21d力竭游泳方式造成运动性疲劳大鼠模型。温针组取蒙医"顶会"和"心穴",使用MLY-Ⅰ型蒙医疗术温针仪进行温针(100mA,40℃),每隔2d治疗1次,每次15min,共7次。在造模第21天检测力竭游泳时间、悬尾不动时间和挣扎次数,采用ELISA方法检测大鼠血清肿瘤坏死因子(TNF-α)、促肾上腺皮质激素(ACTH)和皮质酮含量。结果:模型组与正常组比较,力竭游泳时间明显缩短(P<0.01),悬尾不动时间明显延长(P<0.05),挣扎次数明显减少(P<0.01);温针组与模型组比较,力竭游泳时间明显延长(P<0.05),悬尾不动时间明显缩短(P<0.05),挣扎次数两组间差异无显著性意义(P>0.05)。模型组与正常组比较,TNF-α、ACTH和皮质酮含量均显著升高(P<0.01);温针组与模型组比较,TNF-α、ACTH和皮质酮含量均显著降低(P<0.01,0.05)。结论:蒙医温针可能通过降低运动性疲劳大鼠血清TNF-α、ACTH和皮质酮的含量,调节疲劳状态下亢进的HPA轴和免疫功能,从而改善大鼠运动能力,发挥抗疲劳作用。
Objective To observe the effect of electro-warmed needle （EWN, of Inner-Mongolian medicine） on fatigue rats＇ behavior, hypothalamic-pituitary-adrenocortex （HPA） axis activity and immune system so as to reveal its neuro-endocrinoimmune mechanism. Methods Male SD rats were randomized into control （ n = 20）, model （ n = 20） and EWN （ n = 19） groups. Fatigue model was established by forcing the rat to swim in a water pool till exhaustion, once daily, continuously for 21 days. ＂Dinghui＂ （central spot over the bregmatic bone） and ＂Xinxue＂ （the center of the depression beneath the 7th thoracic vertebra） were punctured with silver needles which were warmed electrically by using a MLY- Ⅰ Electrical Needle-warming Apparatus, once every 3 days, 7 sessions altogether. On the 21^st day of modeling, swim-exhaustion duration （SED）, and immobility time and struggle times in tail suspension test were measured. Twenty-four hours after the last swim, the rats＇ serum TNF-α, ACTH and corticosterone contents were detected by enzyme linked immunosorbent assay （ELISA）. Results Compared with control group, SED, immobility time and struggle times in tail suspension test in model group decreased, increased and lowered respectively and significantly （P〈0.01, 0.05）; while in comparison with model group, the first 2 indexes of EWN group increased and lowered respectively and significantly （P〈0.05）. No significant difference was found between EWN and model groups in struggle times （ P〉0. 05）. Compared with control group, serum TNF-α, ACTH and corticosterone contents in model group increased significantly （P〈0. 01）, while in comparison with model group, the 3 indexes of EWN group were significantly lower （P〈0. 01, 0.05）. Conclusion EWN treatment can reduce fatigue-induced increase of serum TNF-α, ACTH and corticosterone levels, and raise motor ability, suggesting a favorable regulation of HPA axis and immune function after EWN and improvement of fatigue in fatigue rats
出处
《针刺研究》
CAS
CSCD
2008年第4期258-261,共4页
Acupuncture Research
基金
国家自然科学基金资助(30572462)
内蒙古卫生厅资助(2006016)
