摘要
目的:了解南京地区多重耐药鲍曼不动杆菌临床分离株的整合子、转座子存在状况。方法:自2007年7月～10月间南京地区住院患者标本中分离出20株多重耐药鲍曼不动杆菌,微量肉汤稀释法测定11种抗菌药物的敏感性;以PCR法测定整合子遗传标记基因qacE△1-sul1及转座子遗传标记基因tnpU。结果:20株AB菌中整合子qacE△1-sul1基因阳性率70%;转座子tnpU基因阳性率65%。结论:南京地区多重耐药鲍曼不动杆菌整合子、转座子遗传标记基因(qacE△1-sul1、tnpU)携带率高;氯己定预防术后院内感染在我国需要重新评估。
Objective：To study the existence status of the integron and transposable element of multi-resistant Acinetobactor bauman- nii isolates in clinic in Nanjing. Methods：The samples of 20 multi-resistant acinetobacter baumannii isolates were collected from Jul, 2007 to Oct, 2007 of patients in hospitals of Nanjing. To determine the sensitivity to the 11 antibacterial a broth induction method was used, the genetic marker of integron qacE A 1-sull and transposable element tnpU were analyzed by polymerase chain reaction （PCR）. Results：In the 20 AB isolates, the rate of the positive of integron qacEA 1-sull is 70%, and the rate of transposable element tnpU is 65%. Conclusion：The positive rate of the integron qacE △1-sull and transposable element tnpU for muhi-resistant acinetobactor baumannii is high in Nanjing region. It should be reevaluated the chlorhexidine used as precaution infection drug after operation.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第7期876-880,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
南京医科大学重点研究基金(06NMUZ017)
关键词
鲍曼不动杆菌
整合子
转座子
抗药性
多药
Acinetobacter baumanii
integron
transposable element
drug resistance
multiple
