摘要
借助Design-expert统计系统,采用中心组合试验法对真姬菇菌丝体多糖的碱提取工艺进行了优化研究。结果表明,以提取温度、提取时间、液料比、乙醇用量四因素为自变量,以多糖提取率为因变量,回归得到二次多项式模型极显著,模型校正决定系数为0.9495,相关系数为0.9847。当提取温度为73～76℃、提取时间为2.6～3.5h、液料比为18.25～25mL/g、乙醇用量为提取液体积的2.5～3.5倍时,多糖提取率最低为4.45%。当提取温度为76℃、提取时间3.24h、液料比为23mL/g、乙醇用量为提取液体积的3.5倍时,多糖提取率达最高值5.23%。
Based on design-expert statistic system, extraction conditions including temperature, time, the ratio of alkaline solution and mycelium, and the volume ratio of alcohol and alkaline solution were optimized for mycelium polysaccharide by center composition design. The results showed that quadratic model was the best model to describe the relationship between the extraction rate of polysaccharide and the factors. R-squared adjusted value of the model was 0.949 5, and the simulated coefficient R was 0.984 7. By response surface methodology （RSM）, we found that when temperature, time, the ratio of alkaline solution and mycelium, and the volume ratio of alcohol and alkaline solution respectively were73- 76℃, 2.6-3.5 h, 18.25- 25 mL/g and 2.5- 3.5, the lowest extraction rate of polysaccharide was 4.45 %. When temperature, time, the ratio of alkaline solution and mycelium, and the volume ratio of alcohol and alkaline solution separately were 76℃ 3.24 h, 23 mL/g and 3.5, the extraction rate of polysaccharide would reach 5.23 %, a highest value in our investigation extension.
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2008年第6期98-101,93,共5页
Transactions of the Chinese Society for Agricultural Machinery
基金
“十一五”国家科技支撑计划重点项目(项目编号:2006BAK02A24)
关键词
真姬菇
菌丝体多糖
提取工艺
优化
Hypsizigus rnarrnoreus, Mycelium polysaccharide, Extraction technology, Optimization
