摘要
目的建立大鼠高脂血症模型并探讨苜蓿提取物对大鼠高脂血症的影响。方法40只SD大鼠连续饲喂高脂饲料3周,测定血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)水平。根据TC水平将大鼠随机分为高脂模型组和苜蓿提取物低、中、高三个剂量组,各组继续饲喂高脂饲料,各剂量组分别以0.60、1.20、3.60g/kg三个剂量灌服给予苜蓿提取物,另设空白对照组饲喂基础饲料。实验第35天,测定各组大鼠血清TC、TG、HDL-C水平,同时处死动物采集标本用于病理组织学观察。结果大鼠用高脂饲料饲喂3周后,TC、TG、HDL-C水平均升高,给予苜蓿提取物后,TC、TG、HDL-C水平降低。组织病理学观察表明高脂模型组大鼠肝脏存在严重脂肪变性,各剂量组大鼠肝脏病变减轻。结论苜蓿提取物对高脂血症大鼠具有降低TC、TG水平的作用,并可减轻高脂血症大鼠肝脏的病变程度。
Objective To establish hyperlipemia animal model and investigate the functional changes of alfalfa extract in hyperlipemia rats. Methods 40 male SD rats were randomly divided into 4 groups after fed with high-fat and high-cholesterol diet for 3 weeks, The model group（n=10）, fed with high-fat and high-cholesterol diet： the low, middle and high intervention group was added with alfalfa extract at the dosage of 0.60, 1.20 and 3.60 g/kg respectively. Besides other 10 male rats as negative control group fed with normal diet.All the rats were sacrificed after 35 days. The TC, TG, HDL-C levels and the pathological changes of the liver, heart, kidney, lung and artery were detected. Results TC, TG levels were rose after fed with high-fat and high-cholesterol diet and decreased after added with alfalfa extract. Pathological changes lessened in intervention group. Conclusion The alfalfa extract can reduce the TC, TG, HDL-C levels and pathological changes of the liver in hyperlipemia model rat.
出处
《实验动物与比较医学》
CAS
2008年第3期171-173,共3页
Laboratory Animal and Comparative Medicine
