摘要
目的 研究丙烯腈（acrylonitrile，ACN）对大鼠神经胶质正常细胞（DITNC1）和大鼠神经胶质肿瘤细胞（c6细胞）的生长、凋亡、增殖及相关基因表达的影响。方法ACN作用于DITNC1和C6细胞的浓度是25、50和75μg／ml，对于细胞生长、凋亡和增殖实验，作用时间是24h；对于微点阵（microarray）试验，作用时间分别是4、24h。结果25、50和75μg／mlACN作用DITNCl后，DNA合成指数降低，分别为对照的93．1％、81．3％和74．9％，细胞凋亡指数增加，分别为对照的118％、122％和143％；C6细胞的DNA合成指数和凋亡指数在ACN作用后基本没有变化；cyclin和p53等细胞增殖和凋亡相关基因的表达也有一定的变化。结论ACN抑制了DITNC1细胞的增殖和促进了其凋亡，对c6细胞的凋亡和增殖没有变化。
Objective To study the effect of acrylonitrile （ACN） to cell growth, apoptosis, proliferation and related gene expression of rat normal glial cells （ DI TNC1 ） and tumor glial cells （ C6 ）. Methods The concentration of ACN on DI TNC1 and C6 were 25, 50 and 75μg/ml. For cell growth, proliferation and apoptosis assay, the treated time was 24 hours, for microarray assay, the treated time was 4 and 24 hours. Results After treatment of DI TNC1 cell with 25,50 and 75μg/ml ACN, the DNA synthesis index were decreased 93. 1% , 81.3% and 74. 9% as compared to control respectively, the apoptosis index was increased 118%, 122% and 143% as compared to controls respectively. The DNA synthesis and apoptosis indexes of C6 cell showed no change after treatment with ACN. The cell cycle and apoptosis pathway related genes, such as cyclin and p53, also showed changes after treatment with ACN. Conclusion ACN inhibited the cell proliferation of DI TNC1, induced the apoptosis of DI TNC1 and had no effect on cell proliferation and apoptosis of C6 cells, and the related regulation gene expression changes further confirmed the results.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2008年第6期405-409,共5页
Chinese Journal of Preventive Medicine
基金
国家自然科学基金（30400357）
