摘要
背景与目的:有研究表明自噬活性的改变与肿瘤的发生、发展有关。诱导自噬性细胞死亡已经成为杀死肿瘤细胞的新策略。本研究检测微管相关蛋白LC3和自噬基因Beclin1在不同卵巢肿瘤组织中的表达情况,探讨其与上皮性卵巢癌发生、发展的相关性及意义。方法:用免疫组化法检测25例良性卵巢肿瘤、25例交界性卵巢肿瘤及75例上皮性卵巢癌组织的LC3和Beclin1表达,并结合临床病理因素进行分析。结果:良性肿瘤组LC3和Beclin1的阳性率(100%、100%)和交界性卵巢肿瘤组LC3和Beclin1的阳性率(96%、84%)都明显高于上皮性卵巢癌组(57%、57%),且差异具有统计学意义(P均<0.001)。LC3在上皮性卵巢癌中的表达与FIGO分期和肿瘤分化程度相关(P=0.017;0.001)。Beclin1表达与上皮性卵巢癌患者FIGO分期相关(P=0.04)。LC3和Beclin1在卵巢上皮癌中的表达无相关性(P=0.875)。结论:LC3和Beclin1在上皮性卵巢癌中的表达下调,自噬活性的改变可能与上皮性卵巢癌的发生、发展相关。
BACKGROUND ＆OBJECTIVE： Some studies have showed that changes of autophagic capacity may be correlated to tumorigenesis and tumor development. This study was to investigate the expression of microtubule-associated protein 1 light chain 3 （LC3） and autophagy-related gene Beclinl in ovarian tumor tissues, and explore their correlations to the tumorigenesis and development of epithelial ovarian carcinoma. METHODS： Expressions of LC3 and Beclinl in 25 specimens of benign ovarian tumor, 25 specimens of borderline ovarian tumor, and 75 specimens of epithelial ovarian carcinoma were detected by immunohistochemistry. The correlations of LC3 and Beclinl expression to the clinicopathologic characteristics of the 75 epithelial ovarian cancer patients were analyzed. RESULTS： The positive rates of LC3 and Beclin1 were significantly higher in benign and borderline ovarian tumors than in epithelial ovarian carcinoma （100% and 96% vs. 57%, P〈0.001; 100% and 84% vs. 57%, P〈0.001）. The expression of LC3 was associated with FIGO stage and histological grade （P=0.017;0.001）. The expression of Beclin1 was related to FIGO stage （P=0.04）. The expression of LC3 was not correlated to Beclinl （P=0.875）. CONCLUSIONS： Expressions of LC3 and Beclin1 are down-regulated in epithelial ovarian cancer tissues. The decrease of autophagic capacity may relate to tumorigenesis and the development of epithelial ovarian cancer.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2008年第6期595-599,共5页
Chinese Journal of Cancer
