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新生期反复惊厥对海马Beclin-1急性期表达的影响及干预 被引量：4

Expression of Beclin-1 following recurrent neonatal seizures in the hippocampus and intervention by 3-methyladenine
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摘要 目的研究新生期大鼠反复惊厥后海马中Beclin-1急性期的表达及3-甲基腺嘌呤f3-MA）的干预作用。方法生后6d的SD大鼠108只按照随机数字表法分成3组，每组36只，惊厥组每日吸入三氟乙醚诱导惊厥发作5次，每次间隔30min，连续9d；对照组同样操作但不吸人三氟乙醚；3-MA组惊厥前腹腔注射3-MA（总量2μL），然后以同样方法吸入三氟乙醚诱导惊厥，方法同惊厥组。3组大鼠于最后一次惊厥后1．5h、3h、6h、12h、24h、48h断头取脑，每组每个时间点分别为6只大鼠，采用Western blot分别检测3组大鼠大脑海马中Beclin-1的表达。结果与对照组同一时间点相比，惊厥组海马组织中Beclin-1的表达明显增多，差异均有统计学意义（P〈0．05）。与惊厥组同一时间点相比，3-MA组Beclin-1的表达明显减少，差异均有统计学意义（P〈0．05）。结论惊厥后自噬途径被激活，3-MA参与了自噬途径的调控。 Objective To study the role of autophagy/lysosomal pathway in developmental seizure-induced apoptosis of the hippocampal cells, we investigated the dynamic expression of Beclin-1 in the hippocampus and the intervention effect of autophagy/lysosome inhibitor 3-methyladenine （3-MA） on Beclin-1 expression following recurrent seizures in neonatal rats. Methods One hundred and eight SD rats aged 6 d were randomly divided into 3 groups： repeated neonatal seizures （RS group）, 3-MA-treated seizure （3-MA group） and the control grous（n=36）. Rats in the RS group were performed the indraft of flurothyl 5 times daily for a consecutive 9 d. In the 3-MA group, 3-MA was injected every day with a total dosage of 2 μL before seizures were induced. The rats in the 3 groups were sacrificed at 1.5, 3, 6, 12, 24 and 48 h after the last convulsion with 6 rats at each time points; Western blotting was employed to determine the expression of Beclin-1 in the hippocampus at these different time points. Results The expression of Beclin-1 in the RS group was significantly higher than that in the control group at the same time points （P〈0.05）. The expression of Beclin-1 at each time point （1.5, 3, 6, 12, 24 and 48 h） in the 3-MA group was significantly decreased compared with that in the RS group （P〈0.05）. Conclusion Autophagy/lysosomal pathway are activated immediately after the neonatal seizures and 3-MA involves in the regulation of autophagy/lysosomal pathway by down-regulating the expression of Beclin- 1.
作者 倪宏 巩勇 陶陆阳
机构地区 苏州大学附属儿童医院儿科研究所 苏州大学衰老与神经疾病实验室
出处 《中华神经医学杂志》 CAS CSCD 北大核心 2010年第2期124-127,共4页 Chinese Journal of Neuromedicine
基金 基金项目：国家自然科学基金（30870808） 江苏省自然科学基金青年科技创新人才项目（BK2007509）
关键词 惊厥 新生期 BECLIN-1 自噬 3-甲基腺嘌呤 Seizure Neonatal Beclin-1 Autophagy 3-methyladenine
分类号 R742.1 [医药卫生—神经病学与精神病学]
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参考文献18

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共引文献79

同被引文献47

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引证文献4

二级引证文献10

中华神经医学杂志
2010年 第2期

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