摘要
利用2个陆地棉种质材料爱字棉1517与德州047构建了重组近交系。田间调查显示,两亲本田间性状差异多数达到显著或极显著,群体性状数据完全符合受多位点控制的数量性状遗传的特征。分子标记多态性筛选结果表明,两亲本亲缘关系相对较近,利用SSR构建高密度遗传连锁图有一定困难,但对于所定位的QTL位点,其准确性将会有一定程度提高。试验构建了一张包括51个标记分为15个连锁群的遗传连锁图,总长504.05cM,覆盖棉花基因组总长度的10.08%。利用两年田间数据检测到QTL位点15个,其中生育期性状3个,纤维品质性状7个,产量性状5个。与标记图距在1cM以内的QTL位点7个,这将对分子标记辅助育种具有一定的参考价值。
Two typical upland cotton （Acala1517 and Dezhou 047） were applied to construction of intraspecific recombinant inbred lines （RIL）. The agronomic trait of RIL were studied. The trait difference of two parents were significant or extremely significant based on field investigation. The agro nomic trait performances of the RIL completely matched the heredity characters of multi-loci quantita- tive trait. The polymorphism screening result of molecular marker indicated that the genetic relation-ship of two parents was near. So it was difficult to construct the high density linkage map using the RIL. But the RIL should raise the accuracy when it was applied to the gene location study. 51 SSR markers were assigned into 15 linkage groups. The total length of the linkage map is about 504.05 cM, covering 10.08% of the whole cotton genome. 15 QTLs were detected based on two years agro- nomic trait data, including three concerned with earliness, seven with fiber quality and five with yield trait. Seven SSR marker tightly linked with the QTL site were valuable to the marker assisted breeding.
出处
《棉花学报》
CSCD
北大核心
2008年第3期192-197,共6页
Cotton Science
