摘要
目的观察生脉注射液与奥沙利铂联合抗结肠癌肝转移的作用。方法转移性肝癌造模成功后,将72只小鼠随机分为6组(n=12):奥沙利铂组(A组)、生脉中剂量组(B组)、奥沙利铂+生脉小剂量组(C组)、奥沙利铂+生脉中剂量组(D组)、奥沙利铂+生脉大剂量组(E组)和对照组。生脉注射液小、中、大剂量浓度分别为10.0、100.0、1 000.0μg/L,对照组给予生理盐水,0.1 mL/d腹腔注射,连续给药。奥沙利铂剂量浓度为9.3μg/L。给药14 d后处死小鼠,观察瘤重抑制率和肝转移抑制率;采用ELISA法检测小鼠血清白介素(IL)-15、IL-6、血管内皮生长因子(VEGF)和肝细胞生长因子(HGF)水平;免疫组化检测微血管密度(MVD)和VEGF的表达。结果与对照组比较,A、C、D、E组小鼠的瘤重抑制率和肝转移抑制率均明显升高(P<0.01),HGF、IL-15、IL-6和VEGF的水平均明显降低(P<0.05),肝脏MVD明显降低(P<0.01)。A、D、E组VEGF的表达与对照组比较,差异有统计学意义(P<0.05)。结论生脉注射液联合奥沙利铂具有明显的抑制肿瘤生长和抗肿瘤肝转移作用,其对肝转移的干预机制可能与抗肿瘤血管生成有关。
Objective To observe the effects of Shengmai injection combined with Oxaliplatin in the treatment of liver metastasis from colon cancer. Methods Seventy-two Colon-26 cancer cell bearing mice were randomly divided into six groups： Oxaliplatin group （group A）, Shengmai group （group B）, Shengmai injection （low, medium and high dose） combined with Oxaliplatin groups （ group C, group D and group E） and control group （ n -- 12）. The concentration of Oxaliplatin was 9.3 ug/L. The concentration of Shengmai injection in group B, group C, Group D and Group E were 100. 0, 10.0, 100.0 and 1 000.0 ug/L, respectively. The mice in control group were injected with normal saline at 0.1 mL/d. After 14 d, all the mice were sacrificed. The tumor inhibiting rates were obtained, the levels of serum IL-15, IL-6, vascular endothelial growth factor （VEGF） and hepatocyte growth factor （HGF） were measured by ELISA, and microvessel density （MVD） and VEGF expression were determined by immunohistochemical examination. Results Compared with control group, the tumor inhibiting rates were significantly increased, levels of HGF, IL-15, IL-6 and VEGF significantly decreased （P 〈0.05） , and liver MVD significantly decreased（P 〈 0.01 ） in group A, group C, group D and group E. The VEGF expression of group A, group D and group E was significantly different from that of control group（ P 〈 0.05）. Conclusion Shengmai injection can enhance the anti-tumor effect of Oxaliplatin, and the mechanism may be related to the inhibition of angiogenesis.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2008年第5期552-555,共4页
Journal of Shanghai Jiao tong University:Medical Science
关键词
生脉注射液
奥沙利铂
结肠肿瘤
肝转移
Shengmai injection
Oxaliplatin
colon neoplasms
liver metastasis
