摘要
20只22日龄SPF雄性SD大鼠,随机分为对照组和试验组(双酚A染毒组),每组10只。试验组给按5 mg/kg体重双酚A(BPA)灌胃,对照组按等体积植物油灌胃,1次/d。灌胃28 d后,称量大鼠体重;无痛处死后剖检,取睾丸,称量睾丸湿重并计算睾丸系数;采用石蜡切片技术,观察睾丸、附睾的组织病理学变化;采用免疫组织化学方法检测睾丸和附睾中Bc1-2及Bax蛋白表达量的变化。结果显示:BPA染毒组睾丸重量和睾丸系数明显低于对照组(P(0.01);组织病理学观察可见,BPA染毒组睾丸曲细精管内生精细胞和支持细胞明显变性坏死,附睾中精细胞退变、液化。免疫组织化学观察发现BPA染毒组睾丸和附睾中Bax蛋白表达量明显高于对照组(P(0.05),而Bc1-2蛋白表达量却明显低于对照组(P(0.05),Bc1-2/Bax也显著低于对照组(P(0.01)。上述结果表明BPA可引起SPF雄性SD大鼠生殖器官睾丸和附睾的严重的不可逆损伤,造成损伤的机制一方面是直接引起生精细胞和精细胞的坏死液化,另一方面还可通过促进Bax蛋白的表达,同时抑制Bcl-2蛋白的表达以致引起生殖细胞的大量凋亡,结果导致睾丸和附睾结构的严重损伤。
20 male SD rats were divided into 2 groups randomly including normal saline and Bisphenol A （BPA） group. The BPA group rats were export to BPA by intragastric administration at dose of 5.00 mg/kg for 4 weeks. After exposure, the body weight, testis wet weights, ratios of testis/body weight were determined. The histological observation of the testis and epididymis was carried out. The expression of Bcl-2 and Bax in rat spermatogenic cells were measured by immunohistochemical method. Compared with the control group, testis wet weights, the ratio of testis/weight obviously reduced（P 〈 0.01 ）. Optical microscopy showed the number of spermatozoon, spermatocyte and sperm cell was all reduced,the number of necrosis cells in testis and epididymis was increased, and the number of sperms in the epididymal duct were reduced. Meanwhile, Bcl-2 expression was significantly decreased while Bax expression was increased in BPA exposure groups （ P 〈 0. 01 ）. The ratio of Bcl-2/Bax was remarkably reduced. The results suggested that BPA could damage the reproductive system of male mice. The mecha- nism is that BPA induced necrosis of the germ cell in testis and epididymis , and downregulated expression of Bcl-2, upregulated expression of Bax of germ cell.
出处
《科学技术与工程》
2008年第7期1673-1677,共5页
Science Technology and Engineering
基金
国家自然科学基金(3027099530471301)资助
