摘要
借助正交回归多项式建立数学模型的方法,研究了NAA对东方百合＂蒂伯＂（Tiber）鳞片诱导小鳞茎及2,4-D对小叶片诱导愈伤组织的影响,以探讨东方百合组织培养中NAA及2,4-D的用量范围及最佳用量。结果表明,NAA诱导离体鳞片外植体产生小鳞茎的用量范围为0.15～0.9 mg·L^-1,最佳用量为0.69 mg·L^-1;2,4-D诱导叶片形成愈伤组织的最佳用量为3.2 mg·L^-1。
Adopting orthogonal polynomial regression method, the mathematical model of the effects of NAA on bulbil induction, and that of 2,4-D on callus induction from leaflet on oriental lily cv. ＂Tiber＂ were established. The results showed that NAA at 0. 15～0. 9 mg·L^-1 was appreciate, and 0. 69 mg·L^-1 was the optimum concentration for bulbil induction from scale explants in vicro; and that 3.2mg·L^-1 was the optimum concetration of 2,4-D for callus induction from leaflet explant in vitro.
出处
《西北林学院学报》
CSCD
北大核心
2008年第2期83-86,共4页
Journal of Northwest Forestry University
基金
农业部新技术引进项目(2004-z22)
